Sietsema W K, DeLuca H F
J Nutr. 1982 Aug;112(8):1481-9. doi: 10.1093/jn/112.8.1481.
A method is described for measuring the relative activities of vitamin A active substances based on their direct effect on the cornified vaginal epithelium of vitamin A-deficient rats. The results obtained agree well with those found in the tracheal organ culture assay. The relative activities found for several test compounds were: all-trans-retinoyl-beta-glucuronide greater than all-trans-retinoic acid greater than all-trans retinol greater than all-trans-5,6-epoxyretinoic acid. The assay is simple and inexpensive to perform, and should find use in laboratories where the equipment and personnel required for the tracheal organ culture assay are not available.
描述了一种基于维生素A活性物质对维生素A缺乏大鼠的角化阴道上皮的直接作用来测量其相对活性的方法。所得结果与气管器官培养试验中发现的结果非常吻合。几种测试化合物的相对活性为：全反式视黄酰-β-葡萄糖醛酸酯＞全反式视黄酸＞全反式视黄醇＞全反式-5,6-环氧视黄酸。该测定方法操作简单且成本低廉，在没有气管器官培养试验所需设备和人员的实验室中应会有用。
