Miller D A, DeLuca H F
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6419-22. doi: 10.1073/pnas.82.19.6419.
All-trans-retinoic acid is metabolized to a less polar metabolite in rat liver microsomes. This metabolite was proven to be ethyl retinoate by cochromatography on high-performance liquid chromatography, base hydrolysis to all-trans-retinoic acid, and gas chromatography/mass spectral analysis. The formation of ethyl retinoate is a specific enzymatic process; the apparent Km for all-trans-retinoic acid is 9.8 microM. The production of ethyl retinoate is greatly stimulated by the addition of coenzyme A, suggesting the formation of a retinoic acid-coenzyme A intermediate (retinoyl-coenzyme A).
全反式维甲酸在大鼠肝脏微粒体中代谢为极性较小的代谢产物。通过高效液相色谱共色谱法、碱水解为全反式维甲酸以及气相色谱/质谱分析，证明该代谢产物为维甲酸乙酯。维甲酸乙酯的形成是一个特定的酶促过程；全反式维甲酸的表观 Km 为 9.8 μM。添加辅酶 A 可极大地刺激维甲酸乙酯的产生，这表明形成了维甲酸 - 辅酶 A 中间体（视黄酰 - 辅酶 A）。
