Woods D F, Poodry C A
Dev Biol. 1983 Mar;96(1):23-31. doi: 10.1016/0012-1606(83)90307-x.
Treatment of several Drosophila cell lines with the molting hormone (20-hydroxyecdysone) resulted in biochemical and cellular changes including the morphogenetic process of cell aggregation. Radiolabeling of the cell surface proteins revealed 34 polypeptides that are modulated by the hormone's action. This modulation included both expression of "new" proteins and disappearance of preexisting polypeptides. Whereas most of the hormone-induced proteins were lentil lectin-binding glycoproteins, only one group of disappearing proteins appears to bind lentil lectin. Labeling of the cell surface prior to hormone addition revealed no specific modification of preexisting surface proteins which could account for the protein changes observed with one possible exception. The potential relationship between the modulation in surface proteins and the increase in cell-cell adhesion that occurs during hormone exposure is discussed.
用蜕皮激素（20-羟基蜕皮酮）处理几种果蝇细胞系，会导致生化和细胞变化，包括细胞聚集的形态发生过程。对细胞表面蛋白进行放射性标记，发现有34种多肽受激素作用调节。这种调节包括“新”蛋白的表达和原有多肽的消失。虽然大多数激素诱导的蛋白是扁豆凝集素结合糖蛋白，但只有一组消失的蛋白似乎能结合扁豆凝集素。在添加激素之前对细胞表面进行标记，未发现原有表面蛋白有特定修饰，而这种修饰可能解释观察到的蛋白变化，只有一个可能的例外。本文讨论了表面蛋白调节与激素作用期间细胞间黏附增加之间的潜在关系。
