Stevens B, Alvarez C M, Bohman R, O'Connor J D
Cell. 1980 Dec;22(3):675-82. doi: 10.1016/0092-8674(80)90543-7.
The addition of physiological concentrations of the arthropod molting hormone 20-hydroxyecdysone results in the cessation of cell division in the Kc cell line of Drosophila melanogaster. Fluorometric mononitoring of the cell cycle reveals that treatment of the cells with hormone for 12 hr causes a G2 arrest. The dose-response curves are in agreement with those obtained for other hormonal effects in both the Kc line and the intact animal. In the continual presence of hormone, cells remain G2-arrested for approximately 100 hr, resuming division by 120 hr. Cells which have responded once to ecdysteroids and subsequently reentered the cell cycle are insensitive to hormonal restimulation. This lack of response has been correlated with, and is probably due to, the loss of ecdysteroid receptors in stimulated cells.
添加节肢动物蜕皮激素20 - 羟基蜕皮酮的生理浓度会导致黑腹果蝇Kc细胞系中的细胞分裂停止。对细胞周期的荧光监测显示，用激素处理细胞12小时会导致G2期停滞。剂量反应曲线与在Kc细胞系和完整动物中获得的其他激素效应的曲线一致。在持续存在激素的情况下，细胞会在大约100小时内保持G2期停滞，到120小时时恢复分裂。曾经对蜕皮类固醇有反应并随后重新进入细胞周期的细胞对激素再刺激不敏感。这种缺乏反应与受刺激细胞中蜕皮类固醇受体的丧失有关，并且可能是由于该受体的丧失所致。
