检测胸苷激酶缺陷型单纯疱疹病毒1型毒株的比较方法
Comparative methods for detection of thymidine kinase-deficient herpes simplex virus type 1 strains.
作者信息
Harmenberg J, Sundqvist V A, Gadler H, Levén B, Brännström G, Wahren B
出版信息
Antimicrob Agents Chemother. 1986 Oct;30(4):570-3. doi: 10.1128/AAC.30.4.570.
Abstract
Four methods for analyzing viral susceptibility to antiviral substances were compared. In two methods viral products were measured: late viral proteins were measured by an enzyme-linked immunosorbent assay and viral DNA was measured by DNA hybridization. Infectious virus was quantified in the other two assays as the number of plaques and the yield of virus. The enzyme-linked immunosorbent assay procedure in our hands detected the smallest amounts (lowest proportions) of thymidine kinase-deficient herpes simplex virus type 1 mixed with wild-type virus. The thymidine kinase-deficient proportion of the herpes simplex virus type 1 isolate increased rapidly in the presence of acyclovir in cell culture.
摘要
比较了四种分析病毒对抗病毒物质敏感性的方法。其中两种方法用于测量病毒产物：通过酶联免疫吸附测定法测量晚期病毒蛋白，通过DNA杂交法测量病毒DNA。在另外两种测定中，以噬斑数量和病毒产量来量化感染性病毒。在我们的实验中，酶联免疫吸附测定法检测到与野生型病毒混合的胸苷激酶缺陷型单纯疱疹病毒1型的量最少（比例最低）。在细胞培养中，在阿昔洛韦存在的情况下，单纯疱疹病毒1型分离株中胸苷激酶缺陷型的比例迅速增加。
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