Carlsson B, Ohlsson K
Acta Otolaryngol. 1983 Jan-Feb;95(1-2):111-6. doi: 10.3109/00016488309130923.
The localization of antileukoprotease was studied immunohistologically in normal middle ear mucosa specimens obtained at autopsy and in chronically inflamed middle ear mucosa specimens obtained at middle ear surgery for chronic otitis media. In the sections of normal as well as in the sections of chronically inflamed middle ear mucosa, antileukoprotease localization was confined to PAS-positive goblet cells of surface epithelium and to PAS-positive goblet-like cells of submucosal glands and crypts, whereas ciliated mucosal cells and stratified squamous epithelial cells were devoid of anti-leukoprotease. In comparison with normal middle ear mucosa, an increased number of goblet cells--and thus an increased number of cells containing antileukoprotease--was present in the chronically inflamed middle ear mucosa. Since antileukoprotease is a potent inhibitor of granulocyte elastase and Cathepsin G, it was concluded that this proliferation of the respiratory epithelium during inflammatory processes in the middle ear indicates an increased activity of the biologic defence system against the action of granulocyte proteases.
采用免疫组织学方法，对尸检获取的正常中耳黏膜标本以及因慢性中耳炎接受中耳手术时获取的慢性炎症中耳黏膜标本中的抗白细胞蛋白酶定位进行了研究。在正常中耳黏膜切片以及慢性炎症中耳黏膜切片中，抗白细胞蛋白酶定位仅限于表面上皮的PAS阳性杯状细胞以及黏膜下腺体和隐窝的PAS阳性杯状样细胞，而纤毛黏膜细胞和复层鳞状上皮细胞则不含抗白细胞蛋白酶。与正常中耳黏膜相比，慢性炎症中耳黏膜中的杯状细胞数量增加，因此含有抗白细胞蛋白酶的细胞数量也增加。由于抗白细胞蛋白酶是粒细胞弹性蛋白酶和组织蛋白酶G的有效抑制剂，因此得出结论，中耳炎症过程中呼吸道上皮的这种增殖表明生物防御系统针对粒细胞蛋白酶作用的活性增强。
