Bell W R, Kessler C M, Townsend R R
Br J Haematol. 1983 Apr;53(4):599-610. doi: 10.1111/j.1365-2141.1983.tb07311.x.
Infusions of either fibrinogen fragment D or fibrinogen fragment E into rabbits were followed by increases in fibrinogen synthesis determined by the rate of incorporation of 75Se-selenomethionine into circulating fibrinogen. The degree of stimulation was proportional to the amount of protein infused. When 4.5 mg of each fibrinogen fragment was administered separately to different groups of animals, fibrinogen fragment D was associated with a fourfold increase in fibrinogen synthesis above that in the control animals compared with 1.5-fold increase induced by fragment E. Fragments D and E were assayed for bound sialic acid, the absence of which facilitates binding, transport and catabolism of many circulating glycoproteins by the liver. Fibrinogen fragment D contained 1.3% sialic acid compared to 1.4% in fragment E. These data indicate conservation of sialic acid during plasmic digestion of fibrinogen. The capacity of these glycopolypeptide fragments to stimulate fibrinogen synthesis appears unrelated to the nearly identical quantities of N-acetyl neuraminic acid found in each fragment.
给兔子输注纤维蛋白原片段D或纤维蛋白原片段E后，通过75Se-硒代蛋氨酸掺入循环纤维蛋白原的速率来测定，发现纤维蛋白原合成增加。刺激程度与注入的蛋白量成正比。当将4.5毫克的每种纤维蛋白原片段分别给予不同组的动物时，与片段E诱导的1.5倍增加相比，纤维蛋白原片段D使纤维蛋白原合成比对照动物增加了四倍。对片段D和E进行了结合唾液酸的测定，许多循环糖蛋白缺乏唾液酸会促进肝脏对其的结合、转运和分解代谢。纤维蛋白原片段D含有1.3%的唾液酸，而片段E含有1.4%。这些数据表明在纤维蛋白原的血浆消化过程中唾液酸得以保留。这些糖多肽片段刺激纤维蛋白原合成的能力似乎与每个片段中发现的几乎相同数量的N-乙酰神经氨酸无关。
