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鸡红细胞染色质结构域长度的估计

Estimation of domain length of chicken erythrocyte chromatin.

作者信息

Ganguly A, Bagchi B, Bera M, Ghosh A N, Sen A

出版信息

Biochim Biophys Acta. 1983 Apr 15;739(3):286-90. doi: 10.1016/0167-4781(83)90103-3.

DOI:10.1016/0167-4781(83)90103-3
PMID:6830807
Abstract

Chromatin of chicken erythrocyte nuclei was extracted by digestion with micrococcal nuclease. The length distribution of the soluble chromatin was determined by gel electrophoresis and electron microscopy. These results were fitted with a theoretical distribution which was an outcome of the domain model proposed by Igo-Kemenes and Zachau (Igo-Kemenes, T. and H.G. Zachau (1977) Cold Spring Harbour Symp. Quant. Biol. 42, 109-118). A domain length of 45 kbp was obtained.

摘要

用微球菌核酸酶消化提取鸡红细胞核染色质。通过凝胶电泳和电子显微镜测定可溶性染色质的长度分布。这些结果与理论分布相拟合,该理论分布是Igo-Kemenes和Zachau提出的结构域模型的结果(Igo-Kemenes, T.和H.G. Zachau (1977) Cold Spring Harbour Symp. Quant. Biol. 42, 109 - 118)。获得的结构域长度为45千碱基对。

相似文献

1
Estimation of domain length of chicken erythrocyte chromatin.鸡红细胞染色质结构域长度的估计
Biochim Biophys Acta. 1983 Apr 15;739(3):286-90. doi: 10.1016/0167-4781(83)90103-3.
2
DNA repeat lengths of erythrocyte chromatins differing in content of histones H1 and H5.组蛋白H1和H5含量不同的红细胞染色质的DNA重复长度
Nucleic Acids Res. 1980 Feb 11;8(3):529-42. doi: 10.1093/nar/8.3.529.
3
Stability and reversibility of higher ordered structure of interphase chromatin: continuity of deoxyribonucleic acid is not required for maintenance of folded structure.间期染色质高级结构的稳定性和可逆性:维持折叠结构不需要脱氧核糖核酸的连续性。
Biochemistry. 1980 Jun 10;19(12):2544-54. doi: 10.1021/bi00553a002.
4
Enrichment of transcribed and newly replicated DNA in soluble chromatin released from nuclei by mild micrococcal nuclease digestion.通过温和的微球菌核酸酶消化从细胞核释放的可溶性染色质中转录和新复制DNA的富集。
Biochim Biophys Acta. 1984 Jun 16;782(2):202-9. doi: 10.1016/0167-4781(84)90025-3.
5
Chicken erythrocyte nucleus contains two classes of chromatin that differ in micrococcal nuclease susceptibility and solubility at physiological ionic strength.鸡红细胞核含有两类染色质,它们在微球菌核酸酶敏感性和生理离子强度下的溶解度方面存在差异。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):5968-72. doi: 10.1073/pnas.78.10.5968.
6
Nuclease sensitivity of active chromatin.活性染色质的核酸酶敏感性
Nucleic Acids Res. 1980 Nov 25;8(22):5143-55. doi: 10.1093/nar/8.22.5143.
7
A comparison of the structure of chicken erythrocyte and chicken liver chromatin.鸡红细胞与鸡肝染色质结构的比较。
Cell. 1976 Dec;9(4 Pt 1):627-32. doi: 10.1016/0092-8674(76)90045-3.
8
Alteration of domain architecture of chicken erythrocyte chromatin.
Biochim Biophys Acta. 1984 Sep 10;782(4):415-21. doi: 10.1016/0167-4781(84)90048-4.
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Chromatin nu bodies: isolation, subfractionation and physical characterization.染色质核小体:分离、亚分级分离及物理特性分析
Nucleic Acids Res. 1976 Dec;3(12):3271-91. doi: 10.1093/nar/3.12.3271.
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Disruption of the typical chromatin structure in a 2500 base-pair region at the 5' end of the actively transcribed ovalbumin gene.在活跃转录的卵清蛋白基因5'端一个2500个碱基对区域内典型染色质结构的破坏。
EMBO J. 1982;1(2):223-30. doi: 10.1002/j.1460-2075.1982.tb01151.x.