Dobrina A, Rossi F
Biochim Biophys Acta. 1983 Apr 5;762(2):295-301. doi: 10.1016/0167-4889(83)90084-8.
Studies on endothelial cells metabolism have been limited by the lack of availability of a procedure for obtaining such cells in quantities adequate for direct in vitro analysis. Viable and well-dispersed endothelial cells in high yield have been obtained from the cavernous bodies of bovine penis. A preliminary characterization of the metabolic properties of the isolated cells has concerned the respiratory activity and some aspects of the carbohydrate metabolism. This study points out the following metabolic characteristics of endothelial cells: (1) a low respiration that is inhibited by high glucose concentrations (Crabtree effect); (2) a very high glycolytic activity in aerobic conditions, with a low Pasteur effect; (3) a very high potential activity of the hexose monophosphate shunt relative to the actual flux in the pathway. The biological relevance of these observations is discussed.
由于缺乏一种能够获取足够数量内皮细胞用于直接体外分析的方法，内皮细胞代谢的研究受到了限制。已从牛阴茎海绵体中获得了高产率的活的且分散良好的内皮细胞。对分离细胞代谢特性的初步表征涉及呼吸活性和碳水化合物代谢的一些方面。本研究指出了内皮细胞的以下代谢特征：（1）低呼吸作用，受高葡萄糖浓度抑制（克奈特效应）；（2）在有氧条件下具有非常高的糖酵解活性，巴斯德效应低；（3）相对于该途径中的实际通量，磷酸己糖途径具有非常高的潜在活性。讨论了这些观察结果的生物学意义。
