Jacobs S R, Wolfson W L, Cheng L, Lewin K J
Cancer. 1983 May 1;51(9):1621-4. doi: 10.1002/1097-0142(19830501)51:9<1621::aid-cncr2820510912>3.0.co;2-w.
Estrogen receptor content of breast lesions was estimated using a fluorescent cytochemical technique and a competitive protein binding assay. Of 48 cancers examined, an equal proportion contained significant quantities of receptor by either method (62.5%). The concordance between methods for individual patients was also 62.5%. A greater proportion (26%) of patients younger than 45 years of age had receptor-positive cancers using the cytochemical method than were found by the biochemical method (10%). Benign breast disease was also studied using the fluorescent cytochemical method. A greater proportion of lesions containing estrogen receptors was found compared with that cited in the literature for the competitive protein-binding assay. Because of the methodologic simplicity of the fluorescent cytochemical method, further study for routine use is indicated.
采用荧光细胞化学技术和竞争性蛋白结合分析法对乳腺病变的雌激素受体含量进行了评估。在所检测的48例癌症中，两种方法检测出含有大量受体的比例相同（62.5%）。两种方法对个体患者检测结果的一致性也为62.5%。采用细胞化学方法检测出雌激素受体阳性癌症的45岁以下患者比例（26%）高于生化方法检测出的比例（10%）。还采用荧光细胞化学方法对乳腺良性疾病进行了研究。与文献中报道的竞争性蛋白结合分析法相比，检测出含有雌激素受体的病变比例更高。由于荧光细胞化学方法在方法学上较为简单，因此建议进一步开展常规应用研究。
