Bolin R B, Stewart D A, Cheney B A, Chapman K W, Tompkins D R
Exp Hematol. 1983 Mar;11(3):226-30.
The ability to harvest large amounts of hematopoietic stem cells from blood would eliminate the more difficult approach of bone marrow harvest. Unfortunately, concentration of stem cells in the blood compartment is less than 1% of their concentration in bone marrow. Attempts to increase harvest of blood stem cells, as assayed by granulocyte progenitor cells (CFUC), have been only partially successful. Our study confirms previous reports that CFUC can be mobilized into the blood compartment in dogs, but this mobilization is rate-limited. Unlike platelets and granulocytes that are effectively harvested during the first blood volume processed by continuous apheresis, effective CFUC harvest begins during the second blood volume (606 +/- 97.9 CFUC/ml), peaks by the third (740 +/- 30 CFUC/ml), and remains constant through five blood volumes processed (700 +/- 272 CFUC/ml). Since blood CFUC concentration falls at the end of five blood volumes processed (40% of initial values), further continuous apheresis would not be effective. Treatment of animals with lithium did not improve CFUC harvest. These results show that apheresis procedures can be developed to a limited extent to increase the harvest of hematopoietic progenitor and stem cells.
从血液中采集大量造血干细胞的能力将消除骨髓采集这种更困难的方法。不幸的是，血液中干细胞的浓度不到骨髓中浓度的1%。通过粒细胞祖细胞（CFUC）检测来增加血液干细胞采集量的尝试仅取得了部分成功。我们的研究证实了之前的报道，即CFUC可以在犬类中动员到血液中，但这种动员受到速率限制。与血小板和粒细胞不同，血小板和粒细胞在通过连续单采处理的第一个血容量期间能被有效采集，而有效的CFUC采集在第二个血容量时开始（606±97.9 CFUC/ml），在第三个血容量时达到峰值（740±30 CFUC/ml），并在处理五个血容量的过程中保持恒定（700±272 CFUC/ml）。由于在处理五个血容量结束时血液CFUC浓度下降（为初始值的40%），进一步的连续单采将无效。用锂治疗动物并没有改善CFUC的采集。这些结果表明，单采程序可以在有限程度上得到发展，以增加造血祖细胞和干细胞的采集量。
