Gerhartz H H, Fliedner T M
Exp Hematol. 1980 Feb;8(2):209-18.
Populations of granulopoietic progenitor cells, as measured by the colony formation technic (CFUc), of canine bone marrow and blood were compared by velocity sedimentation and tritiated thymidine (3H-TdR) induced cell death rates. Blood CFUc were smaller than those of the bone marrow and had a lower proportion in S-phase, as reflected by these methods. CFUc which were mobilized into the peripheral blood by dextran sulfate had unaltered velocity sedimentation and cell death fraction rates. Removal of CFUc from the blood by 24-hour leukapheresis resulted in doubling of 3H-TdR suicide of bone marrow CFUc, whereas that of blood CFUc was not affected. Depending on the degree of CFUc depletion, granulopoietic progenitor cells which were larger than normal appeared in the blood and bone marrow. It is concluded that there is a subpopulation of CFUc within the bone marrow which is ready for discharge into the circulation. These cells are characterized by small size and a lower proportion in S-phase. Some bone marrow/blood barrier retains large CFUc as well as those in active proliferation within the bone marrow. One might speculate that enlarged CFUc in the bone marrow are a subpopulation which normally provides only a small influx to the total CFUc pool.
采用速度沉降法和氚标记胸腺嘧啶核苷（3H-TdR）诱导细胞死亡率，对犬骨髓和血液中通过集落形成技术（CFUc）测定的粒细胞生成祖细胞群体进行了比较。通过这些方法反映出，血液中的CFUc比骨髓中的小，且处于S期的比例较低。经硫酸葡聚糖动员进入外周血的CFUc，其速度沉降和细胞死亡分数率未发生改变。通过24小时白细胞单采术从血液中去除CFUc，导致骨髓CFUc的3H-TdR自杀率加倍，而血液CFUc的自杀率未受影响。根据CFUc的耗竭程度，血液和骨髓中出现了比正常细胞大的粒细胞生成祖细胞。得出的结论是，骨髓中存在一个CFUc亚群，随时准备释放到循环中。这些细胞的特征是体积小且处于S期的比例较低。某种骨髓/血液屏障将大的CFUc以及骨髓中处于活跃增殖状态的CFUc保留在骨髓内。有人可能推测，骨髓中增大的CFUc是一个亚群，通常仅为总CFUc池提供少量流入。
