Dollenmeier P, Eppenberger H M
Experientia. 1983 Apr 15;39(4):347-50. doi: 10.1007/BF01963115.
Chicken muscle cells secrete characteristic proteins when grown in the serum-free and hormonally defined culture medium 'DMN'. The most prominent band detected by gel electrophoresis represents a protein of mol.wt 22,000. Fibroblasts released a mol.wt 16,000 protein and fibronectin (mol.wt 220,000) into the medium. The mol.wt 22,000 protein band resolved in 2 dimensional gels into 2 spots which migrated to the same positions as small heat shock proteins as well as butyrate-inducible proteins (BIP) which can be demonstrated in whole cell extracts after butyrate treatment in the presence of serum. The synthesis and release of the mol.wt 22,000 protein is repressed by supplementing the culture medium with serum but not with chick embryo extract.
鸡肌肉细胞在无血清且含有特定激素的培养基“DMN”中生长时会分泌特征性蛋白质。凝胶电泳检测到的最明显条带代表一种分子量为22,000的蛋白质。成纤维细胞向培养基中释放了一种分子量为16,000的蛋白质和纤连蛋白（分子量为220,000）。分子量为22,000的蛋白质条带在二维凝胶中分离为2个斑点，其迁移位置与小热休克蛋白以及丁酸盐诱导蛋白（BIP）相同，在血清存在的情况下经丁酸盐处理后，可在全细胞提取物中检测到这些蛋白。通过在培养基中添加血清而非鸡胚提取物，可抑制分子量为22,000蛋白质的合成与释放。
