Bice D E, Harris D L, Muggenburg B A
Exp Lung Res. 1980 Mar;1(1):33-41. doi: 10.3109/01902148009057511.
This study evaluates the immune response in individual lung-associated lymph nodes of beagle dogs after immunization in specific lung lobes. A particulate antigen, sheep red blood cells (SRBC), was used for immunization. The numbers of anti-SRBC antibody-forming cells (AFC) in the tracheobronchial and in the mediastinal lymph nodes were determined 5 days after deposition of SRBC in the left or right apical or left or right diaphragmatic lung lobes. Results indicated that translocation of the SRBC antigen to these lymph nodes was basically ipsilateral because larger numbers of antigen-specific AFC were consistently found in lymph nodes from the same side of the lung that received SRBC immunization. Of 16 dogs immunized, only two did not show ipsilateral antigen stimulation of the lung-associated lymph nodes. This variance occurred only in a single lymph node in each dog. Although the SRBC antigen induced AFC predominantly in the ipsilateral lymph nodes, some AFC were also found in the contralateral lymph nodes. The largest numbers of AFC in the contralateral tracheobronchial lymph nodes were found after immunization in the right apical or right diaphragmatic lung lobes.
本研究评估了比格犬在特定肺叶免疫后，其各个肺相关淋巴结中的免疫反应。使用颗粒性抗原绵羊红细胞（SRBC）进行免疫。在将SRBC注入左或右尖叶或左或右膈叶肺叶5天后，测定气管支气管淋巴结和纵隔淋巴结中抗SRBC抗体形成细胞（AFC）的数量。结果表明，SRBC抗原向这些淋巴结的转移基本是同侧的，因为在接受SRBC免疫的肺同侧的淋巴结中，始终能发现数量更多的抗原特异性AFC。在16只免疫的犬中，只有两只未表现出肺相关淋巴结的同侧抗原刺激。这种差异仅在每只犬的单个淋巴结中出现。尽管SRBC抗原主要在同侧淋巴结中诱导AFC，但在对侧淋巴结中也发现了一些AFC。在右尖叶或右膈叶肺叶免疫后，在对侧气管支气管淋巴结中发现的AFC数量最多。
