Townsend R, Stahl P
Biochem J. 1981 Jan 15;194(1):209-14. doi: 10.1042/bj1940209.
A rat liver mannan-binding protein was isolated by affinity chromatography on invertase--Sepharose by a modification of the method of Kawasaki, Etoh & Yamashina [(1978) Biochem. Biophys. Res. Commun. 81, 1018-1024] and by a new method involving chromatography on mannose-Sepharose. The binding protein appears as a single band on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis with an apparent mol.wt. of approx. 30000. Binding of 125I-labelled mannan is saturable and inhibited by mannose, N-acetylglucosamine, or L-fucose but not by galactose or mannose 6-phosphate. Neoglycoproteins containing mannose, N-acetylglucosamine, or L-fucose, but not galactose, are inhibitory. The neoglycoproteins are 10000-fold more effective (based on moles of sugar) than are free monosaccharides as inhibitors. 125I-labelled mannan binding to the binding protein is calcium-dependent.
通过对川崎、江藤和山岸的方法[（1978年）《生物化学与生物物理学研究通讯》81卷，第1018 - 1024页]进行改进，利用转化酶 - 琼脂糖亲和层析法，并采用一种新的涉及甘露糖 - 琼脂糖层析的方法，从大鼠肝脏中分离出一种甘露糖结合蛋白。该结合蛋白在十二烷基硫酸钠/聚丙烯酰胺凝胶电泳上呈现为一条单一的条带，表观分子量约为30000。125I标记的甘露聚糖的结合是可饱和的，并且受到甘露糖、N - 乙酰葡糖胺或L - 岩藻糖的抑制，但不受半乳糖或甘露糖6 - 磷酸的抑制。含有甘露糖、N - 乙酰葡糖胺或L - 岩藻糖而非半乳糖的新糖蛋白具有抑制作用。作为抑制剂，新糖蛋白（基于糖的摩尔数）的效力比游离单糖高10000倍。125I标记的甘露聚糖与结合蛋白的结合是钙依赖性的。
