Zilberstein A, Ruggieri R, Korn J H, Revel M
EMBO J. 1986 Oct;5(10):2529-37. doi: 10.1002/j.1460-2075.1986.tb04531.x.
Induced human fibroblasts produce several mRNAs encoding interferon (IFN) activity. We previously cloned cDNA for a 1.3-kb RNA designated IFN-beta 2 and distinct from the 0.9-kb IFN-beta 1 mRNA. In vitro transcription--translation mapping of the full-length IFN-beta 2 cDNA sequence, shows that it encodes a 23.7-kd protein of 212 amino acids. This cDNA, fused to the SV40 early gene promoter, was transfected and amplified in Chinese hamster ovary cells and clones were obtained which constitutively produce human interferon activity. Two IFN-beta 2 genomic clones were isolated and their expression in hamster and mouse cells also produces biologically active rIFN-beta 2. Specific immunoassays show that IFN-beta 2 secreted by DNA-transformed rodent cells is a processed 21-kd protein, whose activity is cross-neutralized by antibodies to human IFN-beta 1 but not to IFN-alpha or gamma. The immunoassay also demonstrates the induction of IFN-beta 2 secretion by fibroblasts in response to growth-regulatory cytokines, such as interleukin-1 and tumor necrosis factor. The function of this IFN-beta 2 as an autoregulatory inhibitor of cell growth is discussed.
诱导的人成纤维细胞产生几种编码干扰素（IFN）活性的mRNA。我们先前克隆了一个1.3kb RNA的cDNA，命名为IFN-β2，它与0.9kb的IFN-β1 mRNA不同。对全长IFN-β2 cDNA序列进行体外转录-翻译图谱分析表明，它编码一个由212个氨基酸组成的23.7kd蛋白。将该cDNA与SV40早期基因启动子融合，转染到中国仓鼠卵巢细胞中进行扩增，获得了组成性产生人干扰素活性的克隆。分离出两个IFN-β2基因组克隆，它们在仓鼠和小鼠细胞中的表达也产生具有生物活性的rIFN-β2。特异性免疫分析表明，DNA转化的啮齿动物细胞分泌的IFN-β2是一种经过加工的21kd蛋白，其活性可被抗人IFN-β1的抗体交叉中和，但不能被抗IFN-α或γ的抗体中和。免疫分析还证明，成纤维细胞在受到生长调节细胞因子如白细胞介素-1和肿瘤坏死因子刺激时会诱导IFN-β2的分泌。本文讨论了这种IFN-β2作为细胞生长的自动调节抑制剂的功能。
