Wasmuth J J, Hill J M, Vock L S
Somatic Cell Genet. 1980 Jul;6(4):495-516. doi: 10.1007/BF01539152.
We have isolated a number of emetine-resistant mutants from several different clones of CHO and CHL cells. Protein synthesis in extracts derived from each of the mutants is much more resistant to emetine than in the parental, emetine-sensitive cell lines, indicating the lesions affect the protein synthetic machinery directly. However, hybrid cell lines, derived from fusing either of two different emetine-resistant CHO mutants with either one of two different emetine-resistant CHL cells, are much more sensitive to growth inhibition by emetine than either parent. In addition, the incorporation of [3H] amino acids into protein in vivo and protein synthesis in vitro in extracts derived from these hybrids is much more sensitive to emetine inhibition than in either emetine-resistant parent. In contrast, no complementation was observed in hybrids derived from fusing two emetine-resistant CHO mutants or in hybrids derived from fusing two emetine-resistant CHL cell lines. These results indicate the CHO emetine-resistant mutants belong to one complementation group and the CHL emetine-resistant mutants belong to another. The genetic loci represented by these two complementation groups must both encode for gene products involved in protein synthesis.
我们已经从CHO和CHL细胞的几个不同克隆中分离出了一些抗吐根碱突变体。每个突变体提取物中的蛋白质合成比亲本的、对吐根碱敏感的细胞系对吐根碱更具抗性，这表明损伤直接影响蛋白质合成机制。然而，通过将两种不同的抗吐根碱CHO突变体中的任何一种与两种不同的抗吐根碱CHL细胞中的任何一种融合而获得的杂交细胞系，对吐根碱抑制生长的敏感性比任何一个亲本都要高得多。此外，这些杂交体提取物中[3H]氨基酸在体内掺入蛋白质以及体外蛋白质合成，比任何一个抗吐根碱亲本对吐根碱抑制都更敏感。相反，在融合两个抗吐根碱CHO突变体获得的杂交体或融合两个抗吐根碱CHL细胞系获得的杂交体中未观察到互补作用。这些结果表明，CHO抗吐根碱突变体属于一个互补群，CHL抗吐根碱突变体属于另一个互补群。这两个互补群所代表的遗传位点都必须编码参与蛋白质合成的基因产物。
