Shivanandappa T, Krishnakumari M K, Majumder S K
Poult Sci. 1983 Feb;62(2):405-8. doi: 10.3382/ps.0620405.
Acute oral toxicity of the copper fungicides, copper sulphate and copper oxychloride, were studied in male Gallus domesticus. The median lethal dose for copper sulphate and copper oxychloride determined were 693 and 1263 mg/kg body weight, respectively. Severe diarrhea and delayed mortality (3 to 6 days) was characteristic of copper oxychloride. Liver weights were nearly doubled in birds fed copper oxychloride and marked dose-dependent testicular atrophy was noticed in both copper sulphate and copper oxychloride fed birds. Mild to severe spermatogenetic arrest concomitant with testicular atrophy was observed. Histochemically, the interstitial (Leydig) cells and seminiferous tubules showed intense accumulation of cholesterol-positive lipids. The degree of lipophilia appeared to be directly proportional to the extent of spermatogenetic arrest, and these results are suggestive of steroidogenic inhibition in the Leydig cells of the atrophied testis.
在雄性家鸡中研究了铜杀菌剂硫酸铜和氧氯化铜的急性经口毒性。所测定的硫酸铜和氧氯化铜的半数致死剂量分别为693和1263毫克/千克体重。氧氯化铜的特征是严重腹泻和延迟死亡（3至6天）。喂食氧氯化铜的鸟类肝脏重量几乎翻倍，并且在喂食硫酸铜和氧氯化铜的鸟类中均观察到明显的剂量依赖性睾丸萎缩。观察到轻度至重度的生精停滞并伴有睾丸萎缩。组织化学分析显示，间质（莱迪希）细胞和生精小管中胆固醇阳性脂质大量积聚。亲脂程度似乎与生精停滞程度直接相关，这些结果提示萎缩睾丸的莱迪希细胞中存在类固醇生成抑制。
