Makarova G F, Epifanova O I, Loginov B V
Tsitologiia. 1983 Jan;25(1):39-46.
It has been shown that when cells are incubated for a long time without medium change, imuran exerts a much greater effect on the viability of cells in the late stationary phase of growth than in the early stationary phase. When nutrient medium supplemented with 10% serum was changed for the medium with 0.5% serum, or when a partial change of medium (one third of the volume) was carried out daily from day 4, these differences were only slightly pronounced. Thus, the degree of cell response to imuran in the early and late stationary phases of growth depends upon the way of cell maintaining in the resting state.
已经表明，当细胞在不更换培养基的情况下长时间培养时，硫唑嘌呤对生长后期静止期细胞活力的影响比对早期静止期细胞的影响要大得多。当将补充有10%血清的营养培养基更换为含有0.5%血清的培养基时，或者从第4天开始每天进行部分培养基更换（体积的三分之一）时，这些差异仅略微明显。因此，细胞在生长早期和晚期静止期对硫唑嘌呤的反应程度取决于细胞在静止状态下的维持方式。
