兔红细胞膜内葡萄球菌α毒素的光标记

Photolabeling of staphylococcal alpha-toxin from within rabbit erythrocyte membranes.

作者信息

Thelestam M, Jolivet-Reynaud C, Alouf J E

出版信息

Biochem Biophys Res Commun. 1983 Mar 16;111(2):444-9. doi: 10.1016/0006-291x(83)90326-1.

Abstract

Intrinsic membrane proteins of rabbit red blood cells were labeled with the photoreactive amphipatic reagent 12-(4-azido-2-nitrophenoxy) stearoyl (1-14C) glucosamine, which inserts into the hydrophobic membrane region and generates a reactive nitrene upon ultraviolet irradiation. Photolabeling of membrane-bound staphylococcal alpha-toxin after lysis of probe-treated rabbit red blood cells by this toxin implies its penetration into the hydrophobic region of the outer leaflet of the membrane. In contrast clostridial theta-toxin and staphylococcal delta-toxin were not labeled, but extraction of intrinsic membrane proteins by delta-toxin was evidenced.

摘要

兔红细胞的内在膜蛋白用光反应性两亲试剂12-(4-叠氮基-2-硝基苯氧基)硬脂酰(1-¹⁴C)葡糖胺进行标记，该试剂插入疏水膜区域并在紫外线照射下产生反应性氮烯。在用这种毒素裂解经探针处理的兔红细胞后，对膜结合的葡萄球菌α毒素进行光标记，这表明它渗透到膜外小叶的疏水区域。相比之下，梭菌θ毒素和葡萄球菌δ毒素未被标记，但有证据表明δ毒素可提取内在膜蛋白。