Crum L A, Coakley W T, Deeley J O
Biochim Biophys Acta. 1979 Jun 13;554(1):76-89. doi: 10.1016/0005-2736(79)90008-7.
Heated human erythrocytes gradually lose their form-maintaining structure as the temperature is increased to 50 degrees C and can behave in some respects as a viscous fluid. We have developed a technique for heating and stressing these cells that is novel, simple and quantitatively precise. We have applied this technique to heated human erythrocytes and have measured instability development in cells. We have employed instability growth theory to calculate a value for an effective surface tension which, in contrast to other methods of membrane surface tension measurement sought to minimize the effects of membrane supporting structural elements. The value obtained for the surface tension of the heated erythrocyte membrane was 0.9 . 10(-6) N/m with a range of variation from 0.4 . 10(-6)N/m to 1.4 . 10(-6) N/m. The methods described may be useful for determining fundamental physical parameters such as internal viscosity and interfacial tension in other systems.
随着温度升高至50摄氏度，加热后的人体红细胞会逐渐失去其维持形态的结构，并在某些方面表现得像粘性流体。我们开发了一种加热和对这些细胞施加压力的技术，该技术新颖、简单且定量精确。我们已将此技术应用于加热后的人体红细胞，并测量了细胞中不稳定性的发展情况。我们运用不稳定性增长理论来计算有效表面张力的值，与其他测量膜表面张力的方法相比，该方法试图最小化膜支撑结构元件的影响。加热后红细胞膜的表面张力值为0.9×10⁻⁶ N/m，变化范围为0.4×10⁻⁶ N/m至1.4×10⁻⁶ N/m。所描述的方法可能有助于确定其他系统中的基本物理参数，如内部粘度和界面张力。
