Cisar J O, Vatter A E
Infect Immun. 1979 May;24(2):523-31. doi: 10.1128/iai.24.2.523-531.1979.
Surface antigens of Actinomyces viscosus T14V were released from cell walls by digestion with lysozyme. These were separated by ion-exchange and gel filtration chromatography into fractions rich in carbohydrate or protein. The former contained a polysaccharide high in 6-deoxytalose, along with a peptide fragment from the cell wall. In the protein-rich fractions, material of high molecular weight was present, which contained some carbohydrate and up to 14.3% nitrogen. Aspartic acid, threonine, glutamic acid, lysine, alanine, and glycine were detected in these fractions, along with smaller amounts of 10 other amino acids. Most of the alanine was present as the L isomer and thus was not from peptidoglycan. Electron microscopy of the high-molecular-weight material revealed long fibrils, 3.5 to 4.5 nm in diameter, which resembled those seen on bacterial cells. V-specific antiserum, prepared by absorbing anti-A. viscosus T14V serum with cell walls of the avirulent strain (A. viscosus T14AV), did not react with the 6-deoxytalose polysaccharide but reacted well with isolated fibrils, and this was not inhibited by 6-deoxytalose.
黏性放线菌T14V的表面抗原通过溶菌酶消化从细胞壁中释放出来。通过离子交换和凝胶过滤色谱将其分离成富含碳水化合物或蛋白质的组分。前者含有一种6-脱氧塔罗糖含量高的多糖，以及来自细胞壁的一个肽片段。在富含蛋白质的组分中，存在高分子量物质，其含有一些碳水化合物且氮含量高达14.3%。在这些组分中检测到天冬氨酸、苏氨酸、谷氨酸、赖氨酸、丙氨酸和甘氨酸，以及少量其他10种氨基酸。大部分丙氨酸以L-异构体形式存在，因此并非来自肽聚糖。对高分子量物质的电子显微镜观察显示出直径为3.5至4.5纳米的长纤维，类似于在细菌细胞上看到的那些。通过用无毒菌株（黏性放线菌T14AV）的细胞壁吸收抗黏性放线菌T14V血清制备的V特异性抗血清，不与6-脱氧塔罗糖多糖反应，但与分离出的纤维反应良好，且这种反应不受6-脱氧塔罗糖抑制。
