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粘性放线菌T14V和T14AV表面成分的修饰

Modification of surface composition of Actinomyces viscosus T14V and T14AV.

作者信息

Powell J T, Fischlschweiger W, Birdsell D C

出版信息

Infect Immun. 1978 Dec;22(3):934-44. doi: 10.1128/iai.22.3.934-944.1978.

DOI:10.1128/iai.22.3.934-944.1978
PMID:730385
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC422246/
Abstract

The morphology and serology of Actinomyces viscosus T14V and T14AV were compared. When grown in supplemented tryptic soy broth, the virulent strain (T14V) possessed an extensive network of cell surface fibrils. In this medium, the avirulent strain (T14AV) possessed a microcapsule, absent on strain T14V, and a comparatively small number of surface fibrils. Mild acid extraction (Lancefield procedure) solubilized common antigenic components on both strains as well as components detectable only in the virulent strain T14V (virulence-associated antigens 1 and 2). When grown in Socransky chemically defined medium or Carlsson complex medium, the avirulent strain possessed increased amounts of surface fibrils and virulence-associated antigens. Whole cells and extracts of avirulent cells grown in Socransky medium absorbed antibodies to virulence-associated antigens with approximately the same efficiency as did whole cells and extracts of strain T14V, suggesting antigenic similarity between the two cell types. The results strongly support the hypothesis that observable differences between A. viscosus strains T14V and T14AV represent quantitative, rather than qualitative, differences in particular cell surface components. In addition, the magnitude of these differences can be modified by changing growth conditions.

摘要

对粘性放线菌T14V和T14AV的形态学和血清学进行了比较。在添加了胰蛋白酶大豆肉汤的培养基中生长时，有毒力的菌株（T14V）具有广泛的细胞表面纤丝网络。在这种培养基中，无毒力的菌株（T14AV）具有微荚膜，而T14V菌株没有，并且表面纤丝数量相对较少。温和酸提取法（兰斯菲尔德法）可溶解两种菌株上的共同抗原成分以及仅在有毒力的菌株T14V中可检测到的成分（毒力相关抗原1和2）。当在索克兰斯基化学限定培养基或卡尔森复合培养基中生长时，无毒力的菌株表面纤丝和毒力相关抗原的量增加。在索克兰斯基培养基中生长的无毒力细胞的全细胞和提取物与T14V菌株的全细胞和提取物一样，以大致相同的效率吸收针对毒力相关抗原的抗体，这表明两种细胞类型之间存在抗原相似性。结果有力地支持了这样一种假说，即粘性放线菌菌株T14V和T14AV之间可观察到的差异代表特定细胞表面成分的数量差异，而非质量差异。此外，这些差异的程度可以通过改变生长条件来改变。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/f4469f9ea490/iai00204-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/4581a083aad7/iai00204-0306-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/800eb47027c0/iai00204-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/a623b7bd1e94/iai00204-0308-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/afa4157813f5/iai00204-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/4a7bae3fa3a8/iai00204-0309-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/2e52cc0f3e8f/iai00204-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/f4469f9ea490/iai00204-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/4581a083aad7/iai00204-0306-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/800eb47027c0/iai00204-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/a623b7bd1e94/iai00204-0308-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/afa4157813f5/iai00204-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/4a7bae3fa3a8/iai00204-0309-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/2e52cc0f3e8f/iai00204-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7763/422246/f4469f9ea490/iai00204-0311-a.jpg

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