Muller D E, Siegl G
J Gen Virol. 1983 May;64(Pt 5):1043-54. doi: 10.1099/0022-1317-64-5-1043.
The development of an in vitro system prepared by lysis of LuIII virus-infected cells with Brij-58 has enabled the study of the assembly pathway of a parvovirus. Under optimal conditions, radioactive precursors are incorporated both into viral replicative form double-stranded DNA and into progeny viral DNA (vDNA) during pulses as short as 30 s. Labelled 110S particles can be isolated at the end of such pulses. Therefore, synthesis and encapsidation of progeny viral DNA into pre-existing empty viral capsids appear to be closely related processes. Up to about 10 min after incorporation of vDNA, the 110S particles band at 1.44 g/ml and are relatively unstable in 3.5 M-CsCl. Moreover, newly synthesized vDNA molecules show an abnormal electrophoretic behaviour probably due to the presence of a covalently linked terminal protein. This so far uncharacterized alkali-stable polypeptide is lost (cleaved off?) concomitant with the maturation of the 110S virus particles. Maturation is reflected by a change in the virus stability in CsCl and a shift in density from 1.44 to 1.41 g/ml around 10 to 15 min after encapsidation of progeny vDNA.
通过用Brij-58裂解感染了LuIII病毒的细胞制备体外系统，这使得对细小病毒的组装途径的研究成为可能。在最佳条件下，放射性前体在短至30秒的脉冲期间既被掺入病毒复制型双链DNA中，也被掺入子代病毒DNA（vDNA）中。在这种脉冲结束时可以分离出标记的110S颗粒。因此，子代病毒DNA的合成和衣壳化进入预先存在的空病毒衣壳似乎是密切相关的过程。在掺入vDNA后长达约10分钟，110S颗粒在1.44 g/ml处形成条带，并且在3.5 M CsCl中相对不稳定。此外，新合成的vDNA分子表现出异常的电泳行为，这可能是由于存在共价连接的末端蛋白。这种迄今为止未表征的碱稳定多肽伴随着110S病毒颗粒的成熟而丢失（被切割掉？）。成熟表现为在子代vDNA衣壳化后约10至15分钟，病毒在CsCl中的稳定性发生变化，密度从1.44 g/ml转变为1.41 g/ml。
