Diffoot N, Chen K C, Bates R C, Lederman M
Department of Biology, Virginia Polytechnic Institute, Blacksburg 24061.
Virology. 1993 Jan;192(1):339-45. doi: 10.1006/viro.1993.1040.
Parvovirus LuIII encapsidates single-stranded DNA of either plus or minus polarity with equal frequency, whereas the rodent parvoviruses MVMp and H-1 encapsidate minus strand DNA only. A full-length, infectious clone of LuIII was constructed and the complete nucleotide sequence of the genome was determined. Comparison of the LuIII sequence with those of MVMp and H-1 revealed that these viruses are virtually identical with respect to the genomic organization, location of regulatory signals, mRNA splicing patterns, and amino acid sequences of viral proteins. However, two regions of the LuIII sequence differ significantly from those of the rodent parvoviruses. At mu 92, LuIII has only one copy of a sequence found as a direct repeat in MVMp and H-1. Upstream of this sequence, at mu 89, there is an A-T-rich region, 47 nucleotides in length, unique to the LuIII genome. This A-T-rich region could represent a signal responsible for the totally different encapsidation patterns observed for these viruses.
细小病毒LuIII以相同频率包装正链或负链的单链DNA，而啮齿动物细小病毒MVMp和H - 1仅包装负链DNA。构建了LuIII的全长感染性克隆，并确定了基因组的完整核苷酸序列。将LuIII序列与MVMp和H - 1的序列进行比较，发现这些病毒在基因组组织、调控信号位置、mRNA剪接模式以及病毒蛋白的氨基酸序列方面几乎相同。然而，LuIII序列的两个区域与啮齿动物细小病毒的区域有显著差异。在92位，LuIII只有一个在MVMp和H - 1中作为直接重复序列存在的序列拷贝。在该序列上游的89位，有一个富含A - T的区域，长度为47个核苷酸，是LuIII基因组所特有的。这个富含A - T的区域可能代表一个负责这些病毒观察到的完全不同包装模式的信号。
