Chevion M, Navok T
Anal Biochem. 1983 Jan;128(1):152-8. doi: 10.1016/0003-2697(83)90355-x.
A new method for the quantitation of the favism-inducing agents in legumes is described. The procedure involves differential extraction of the glucosides vicine and convicine with acetic acid (25%), followed by an enzymatic hydrolysis by beta-glucosidase under anaerobic conditions. Each of the aglycone moieties, isouramil and divicine, anaerobically reduces two molecules of o-ferriphenanthroline to o-ferrophenanthroline. This reaction is readily followed spectrophotometrically at 515 nm. Using this procedure, it was found that in various strains of Vicia faba, the level of these two glucosides comprises approximately 0.5% of the wet weight of the seeds. In contrast, these glucosides could not be detected in either green peas or chick peas.
描述了一种定量豆类中诱发蚕豆病因子的新方法。该程序包括用25%的乙酸对糖苷蚕豆嘧啶和伴蚕豆嘧啶进行差异提取，然后在厌氧条件下用β-葡萄糖苷酶进行酶促水解。每种糖苷配基部分，异脲嘧啶和双嘧啶，在厌氧条件下将两分子邻菲啰啉铁还原为邻菲啰啉亚铁。该反应可在515nm处通过分光光度法轻松跟踪。使用该程序发现，在不同的蚕豆品种中，这两种糖苷的含量约占种子湿重的0.5%。相比之下，在青豆或鹰嘴豆中均未检测到这些糖苷。
