Dohle G R, Beekhuis J A, van Steenbrugge G J, Schröder F H, Tanke H J
Department of Urology, University Hospital Rotterdam, The Netherlands.
Urol Res. 1996;24(5):251-5. doi: 10.1007/BF00304773.
The value of nuclear protein (NP) as a prognostic parameter in prostatic adenocarcinoma was investigated. The NP and DNA contents of two prostatic tumour lines with a well-documented hormonal dependency (PC-82, PC-EW) were compared to the NP and DNA contents of two xenografts with only partial or no response to androgen deprivation (LNCaP, PC-133). After hormonal treatment the PC-82 and PC-EW tumours showed a significant decrease in the NP/DNA ratio, which coincided with a decrease in the proliferative activity [anti-bromodeoxyuridine (BRDU) antibody-labelling index] of the same specimens. In the fast-growing LNCaP tumour an increased percentage of cells with high NP and DNA contents was found. The tumours PC-82, PC-EW, and PC-133 with lower proliferative activity showed lower nuclear protein and DNA contents. In a pilot study of 20 prostatic biopsies the amount of nuclear protein and DNA in grade 1-3 tumours as well as in dysplasia was measured. Statistically significant differences (P < 0.002) were found between grade 1 and grade 3 tumours. The mean NP/DNA ratio was increased in high-grade malignancies. Nuclear protein appeared to be a potential parameter in predicting growth activity in prostatic carcinoma.
研究了核蛋白（NP）作为前列腺腺癌预后参数的价值。将两个激素依赖性明确的前列腺肿瘤细胞系（PC-82、PC-EW）的NP和DNA含量与两个对雄激素剥夺仅有部分反应或无反应的异种移植瘤（LNCaP、PC-133）的NP和DNA含量进行比较。激素治疗后，PC-82和PC-EW肿瘤的NP/DNA比值显著降低，这与相同标本增殖活性（抗溴脱氧尿苷（BRDU）抗体标记指数）的降低相一致。在快速生长的LNCaP肿瘤中，发现具有高NP和DNA含量的细胞百分比增加。增殖活性较低的PC-82、PC-EW和PC-133肿瘤显示核蛋白和DNA含量较低。在一项对20例前列腺活检的初步研究中，测量了1-3级肿瘤以及发育异常中的核蛋白和DNA含量。1级和3级肿瘤之间存在统计学显著差异（P < 0.002）。高级别恶性肿瘤的平均NP/DNA比值升高。核蛋白似乎是预测前列腺癌生长活性的一个潜在参数。
