Braatz J A, Hua D T, Princler G L
Cancer Res. 1983 Jan;43(1):110-3.
A human lung tumor-associated antigen, previously purified to apparent homogeneity from an extract of a small cell tumor, was radioiodinated with Bolton-Hunter reagent for use in a competitive protein-binding radioimmunoassay. A panel of 215 sera was assembled from normal individuals and pretreatment patients with lung cancer, benign lung disease, and nonlung cancers, and lung tumor antigen in each was quantitated using the radioimmunoassay. The mean of normals was 0.92 +/- 0.43 (S.D.) microgram/ml (n = 88), and values greater than 2 standard deviations above the mean (1.78 micrograms/ml) were considered positive. Positive rates in lung cancers of the following histological types were found: adenocarcinoma, 60% (9 of 15); squamous cell, 42% (13 of 31); large cell, 17% (3 of 18); and small cell, 19% (3 of 16). In addition, 13% (3 of 23) of other cancers, 0% (0 of 24) of benign lung disease, and 2% (2 of 88) of normals were positive. Approximately one-third of Stage 1 patients in the squamous cell and adenocarcinoma groups were positive while two-thirds of patients with more advanced Stage III disease in these categories showed elevations.
一种先前从小细胞肿瘤提取物中纯化至表观均一的人肺肿瘤相关抗原，用博尔顿-亨特试剂进行放射性碘化，用于竞争性蛋白结合放射免疫测定。收集了一组由正常个体以及肺癌、良性肺病和非肺癌的预处理患者组成的215份血清样本，并用放射免疫测定法定量每份样本中的肺肿瘤抗原。正常组的平均值为0.92±0.43（标准差）微克/毫升（n = 88），高于平均值2个标准差（1.78微克/毫升）的值被视为阳性。发现以下组织学类型的肺癌阳性率为：腺癌，60%（15例中的9例）；鳞状细胞癌，42%（31例中的13例）；大细胞癌，17%（18例中的3例）；小细胞癌，19%（16例中的3例）。此外，其他癌症中有13%（23例中的3例）、良性肺病中有0%（24例中的0例）、正常个体中有2%（88例中的2例）呈阳性。鳞状细胞癌和腺癌组中约三分之一的I期患者呈阳性，而这些类型中III期疾病更晚期的患者中有三分之二的人抗原水平升高。
