Parsa I, Kauffman S L
Cancer Lett. 1983 Apr;18(3):311-6. doi: 10.1016/0304-3835(83)90241-0.
Lung adenoma induced in Swiss-Webster mice by transplacental exposure to ethylnitrosourea were minced, cultured and carried as cell lines for up to 2 years. The evolution of cell lines was followed every 3 months by ability to grow in nude mice and by morphological and ultrastructural examination of the tumors. Four cell lines, each originating from a single cell inoculum, were cloned. One of these produced mainly papillary tumors in nude mice and ultrastructurally had the morphology of Clara cells.
通过经胎盘暴露于乙基亚硝基脲在瑞士-韦伯斯特小鼠中诱导产生的肺腺瘤被切碎、培养，并作为细胞系保存长达2年。每隔3个月通过在裸鼠中的生长能力以及肿瘤的形态学和超微结构检查来跟踪细胞系的演变。从单个细胞接种物中分别衍生出四个细胞系并进行克隆。其中一个细胞系在裸鼠中主要产生乳头状肿瘤，超微结构上具有克拉拉细胞的形态。
