Lynch C, Lim C K, Thomas M, Peters T J
Clin Chim Acta. 1983 May 9;130(1):117-22. doi: 10.1016/0009-8981(83)90265-6.
A new method for the assay of blood and tissue acetaldehyde is described. Samples are reacted with a methanolic solution of 2,4-dinitrophenylhydrazine (DNP) and the DNP-aldehyde adducts extracted into CHCl3. DNP-[14C]formaldehyde is added as internal standard. The CHCl3 extracts are washed with HCl and H2O and purified by aluminium oxide chromatography. The eluate is dried down, re-dissolved in methanol and subjected to quantitative analysis by HPLC. The acetaldehyde adduct was identified by co-chromatography with the authentic derivative and by mass spectrometry. Recoveries of added acetaldehyde were 85% and addition of 20 mmol ethanol to the sample gave no apparent increment in acetaldehyde content. This technique is suitable for assessment of acetaldehyde levels in clinical and experimental studies of ethanol metabolism.
本文描述了一种测定血液和组织中乙醛的新方法。样品与2,4-二硝基苯肼（DNP）的甲醇溶液反应，生成的DNP-醛加合物萃取到CHCl3中。加入DNP-[14C]甲醛作为内标。CHCl3萃取物先后用HCl和H2O洗涤，再通过氧化铝柱色谱法纯化。洗脱液浓缩干燥后，重新溶解于甲醇中，然后用HPLC进行定量分析。通过与标准衍生物共色谱分析和质谱分析鉴定乙醛加合物。添加乙醛的回收率为85%，向样品中添加20 mmol乙醇后，乙醛含量无明显增加。该技术适用于乙醇代谢临床和实验研究中乙醛水平的评估。
