腺苷酸能荷对猪心脏和骨骼肌AMP脱氨酶的调节

Regulation of porcine heart and skeletal muscle AMP-deaminase by adenylate energy charge.

Harmsen E, Verwoerd T C, Achterberg P W, De Jong J W

Comp Biochem Physiol B. 1983;75(1):1-3. doi: 10.1016/0305-0491(83)90029-9.

Abstract

Cytosol from pig skeletal muscle, but not heart, contains an inhibitor of AMP-deaminase (AMP-D, EC 3.5.4.6) which reduces AMP-D activity 8-fold. 2. Heart and skeletal muscle AMP-D have been purified to apparent homogeneity by cellulose phosphate and DEAE-Sephacel chromatography. 3. AMP-D from skeletal muscle is inhibited more severely than the heart enzyme by an increase in adenylate energy charge to levels exceeding 0.4. Nevertheless both enzymes seem to be regulated by the energy charge, which contrasts with reports for rabbit heart AMP-D.

摘要

猪骨骼肌的胞质溶胶中含有一种AMP脱氨酶（AMP-D，EC 3.5.4.6）抑制剂，而心肌中则没有，该抑制剂可使AMP-D活性降低8倍。2. 通过磷酸纤维素和DEAE-琼脂糖凝胶柱层析，已将心脏和骨骼肌中的AMP-D纯化至表观均一。3. 当腺苷酸能荷增加到超过0.4时，骨骼肌中的AMP-D比心脏中的酶受到更严重的抑制。然而，两种酶似乎都受能荷调节，这与关于兔心脏AMP-D的报道形成对比。