P388小鼠淋巴瘤细胞中胸苷激酶基因座半合子状态的细胞遗传学证据。

Cytogenetic evidence for hemizygosity at the thymidine kinase locus in P388 mouse lymphoma cells.

作者信息

Yasseen A A, McKenna P G

出版信息

Experientia. 1983 May 15;39(5):532-4. doi: 10.1007/BF01965192.

DOI:10.1007/BF01965192

Abstract

A detailed cytogenetic investigation was carried out on P388 mouse lymphoma cells. The cells have a mean chromosome number of 36.86 with a mode and median of 37 chromosomes. G-banding analysis of 12 spreads revealed a total of 15 marker chromosomes with chromosome 11, the determinant of thymidine kinase, being present only in single copy per cell. It is therefore concluded that the P388 cell line is hemizygous at the thymidine kinase locus. Thymidine kinase activities were assayed in P388 cells and two other malignant cell lines, clone 707 Friend mouse leukaemia cells and L5178Y mouse lymphoma cells. No clear relationship was observed between enzyme activity and gene dosage.

摘要

对P388小鼠淋巴瘤细胞进行了详细的细胞遗传学研究。这些细胞的平均染色体数为36.86，众数和中位数为37条染色体。对12个细胞分裂相进行的G带分析显示共有15条标记染色体，胸苷激酶的决定因素染色体11在每个细胞中仅以单拷贝形式存在。因此得出结论，P388细胞系在胸苷激酶基因座处是半合子。在P388细胞以及另外两种恶性细胞系，即克隆707弗氏小鼠白血病细胞和L5178Y小鼠淋巴瘤细胞中测定了胸苷激酶活性。未观察到酶活性与基因剂量之间有明确关系。