Mayorek N, Bar-Tana J
J Biol Chem. 1983 Jun 10;258(11):6789-92.
The fatty acyl substrate specificity for de novo diglyceride, triglyceride, and phospholipid synthesis in cultured hepatocytes was evaluated. The fatty acyl availability for diglyceride and phospholipid synthesis is restricted to fatty acids longer than myristic acid with octanoic and decanoic acids being excluded. On the other hand, octanoic and decanoic acids may serve as substrates for the diglyceride acyltransferase. Dodecanoic acid appears to occupy an intermediate position between medium and long chain fatty acids with respect to its availability as substrate for the esterification sites concerned. The availability of octanoate as a specific substrate for the diglyceride acyltransferase reaction in cultured hepatocytes enables the measurement of this activity in situ under conditions of overall triglyceride synthesis.
评估了培养肝细胞中从头合成甘油二酯、甘油三酯和磷脂时的脂肪酰基底物特异性。甘油二酯和磷脂合成的脂肪酰基可用性仅限于比肉豆蔻酸长的脂肪酸，辛酸和癸酸被排除在外。另一方面，辛酸和癸酸可作为甘油二酯酰基转移酶的底物。就其作为相关酯化位点底物的可用性而言，十二烷酸似乎处于中链和长链脂肪酸之间的中间位置。辛酸作为培养肝细胞中甘油二酯酰基转移酶反应的特异性底物，使得能够在总甘油三酯合成条件下原位测量该活性。
