Reagan B F, McInerny V K, Treadwell B V, Zarins B, Mankin H J
J Bone Joint Surg Am. 1983 Jun;65(5):629-31.
In an effort to determine the optimum solution for irrigation during arthroscopic procedures, an in vitro metabolic experiment was performed in which cartilage slices were incubated with 35SO4 in various commercially available solutions, harvested at regular intervals, and assayed for incorporated radioactivity. The solutions were compared with Ham F12 medium, a complex, ionically balanced salt and amino-acid solution that is used for tissue culture. The data show that neither normal saline or phosphate-buffered saline supports metabolic activity as well as Ringer lactate or acetate, both of which approximate the values for the control, Ham F12 solution.
Arthroscopy and arthroscopic surgical procedures have become commonplace in orthopaedic practice. Normal saline solution, commonly used in large quantities as an irrigating solution, is in fact not physiological, and we showed that it inhibits normal synthesis of proteoglycan by the chondrocytes. Ringer lactate seemed to support cartilage metabolism as well as an "ideal" tissue-culture medium (Ham F12 solution). Since Ringer solution and normal saline cost the same in our hospital, we strongly recommend that this more physiological solution be used for arthroscopic procedures.
为了确定关节镜手术期间的最佳冲洗液，进行了一项体外代谢实验，将软骨切片置于各种市售溶液中与35SO4一起孵育，定期收集样本，并检测其放射性掺入量。将这些溶液与Ham F12培养基进行比较，Ham F12培养基是一种用于组织培养的复杂的、离子平衡的盐和氨基酸溶液。数据显示，生理盐水或磷酸盐缓冲盐水均不能像乳酸林格液或醋酸林格液那样支持代谢活性，后两者接近对照Ham F12溶液的值。
关节镜检查和关节镜手术在骨科实践中已很常见。大量用作冲洗液的生理盐水实际上并非生理性的，我们发现它会抑制软骨细胞正常合成蛋白聚糖。乳酸林格液似乎能像“理想”的组织培养基（Ham F12溶液）一样支持软骨代谢。由于我院乳酸林格液和生理盐水价格相同，我们强烈建议在关节镜手术中使用这种更具生理性的溶液。
