使用邻苯二甲醛荧光检测的高效液相色谱法分离河豚毒素和麻痹性贝类毒素。

Separation of tetrodotoxin and paralytic shellfish poisons by high-performance liquid chromatography with a fluorometric detection using o-phthalaldehyde.

作者信息

Onoue Y, Noguchi T, Nagashima Y, Hashimoto K, Kanoh S, Ito M, Tsukada K

出版信息

J Chromatogr. 1983 Mar 4;257(2):373-9. doi: 10.1016/s0021-9673(01)88193-0.

Abstract

Tetrodotoxin (TTX) and a variety of paralytic shellfish poisons (PSPs) were extracted from toxic specimens of puffer and scallop, and quantitated by high-performance liquid chromatography with a fluorometric detection using o-phthalaldehyde. Fluorescence spectra for the TTX- and PSP-fluorophors in 0.05 M borate buffer (pH 10) showed maxima at 453 nm with 332-nm excitation. The fluorescence intensity per nM of TTX was found to be 3 and those of PSP to be 4-12. This fluorometric technique may be useful for the simultaneous quantitation of TTX and PSP in small volumes of toxin extracts.

摘要

从河豚和扇贝的有毒样本中提取了河豚毒素（TTX）和多种麻痹性贝类毒素（PSP），并通过高效液相色谱法结合邻苯二甲醛荧光检测进行定量分析。在0.05 M硼酸盐缓冲液（pH 10）中，TTX和PSP荧光团的荧光光谱在332 nm激发下，于453 nm处出现最大值。发现每纳摩尔TTX的荧光强度为3，而PSP的荧光强度为4 - 12。这种荧光检测技术可能有助于对少量毒素提取物中的TTX和PSP进行同时定量分析。