Onoue Y, Noguchi T, Nagashima Y, Hashimoto K, Kanoh S, Ito M, Tsukada K
J Chromatogr. 1983 Mar 4;257(2):373-9. doi: 10.1016/s0021-9673(01)88193-0.
Tetrodotoxin (TTX) and a variety of paralytic shellfish poisons (PSPs) were extracted from toxic specimens of puffer and scallop, and quantitated by high-performance liquid chromatography with a fluorometric detection using o-phthalaldehyde. Fluorescence spectra for the TTX- and PSP-fluorophors in 0.05 M borate buffer (pH 10) showed maxima at 453 nm with 332-nm excitation. The fluorescence intensity per nM of TTX was found to be 3 and those of PSP to be 4-12. This fluorometric technique may be useful for the simultaneous quantitation of TTX and PSP in small volumes of toxin extracts.
从河豚和扇贝的有毒样本中提取了河豚毒素（TTX）和多种麻痹性贝类毒素（PSP），并通过高效液相色谱法结合邻苯二甲醛荧光检测进行定量分析。在0.05 M硼酸盐缓冲液（pH 10）中，TTX和PSP荧光团的荧光光谱在332 nm激发下，于453 nm处出现最大值。发现每纳摩尔TTX的荧光强度为3，而PSP的荧光强度为4 - 12。这种荧光检测技术可能有助于对少量毒素提取物中的TTX和PSP进行同时定量分析。
