Vaca G, Zúñiga P, Medina C, Alonso R, González-Quiroga G, Ortíz-De-Luna R I, Cantú J M
J Clin Pathol. 1983 Jun;36(6):697-700. doi: 10.1136/jcp.36.6.697.
A new fluorimetric method for the quantification of red blood cell (RBC) sorbitol dehydrogenase is described. It is based on the oxidation of sorbitol to fructose, in presence of NAD+, catalysed by the RBC-sorbitol dehydrogenase. The quantity of NADH formed is then measured in a filter fluorimeter. Comparison with an indirect spectrophotometric assay yielded good correlation; however, the present method offers several advantages: it is more rapid, simple and inexpensive. It should be useful to screen for sorbitol dehydrogenase deficiency in large numbers of individuals, particularly patients with diabetes or cataracts.
本文描述了一种用于定量红细胞山梨醇脱氢酶的新荧光测定法。该方法基于在红细胞山梨醇脱氢酶的催化下，山梨醇在NAD⁺存在的情况下氧化为果糖。然后在滤光荧光计中测量生成的NADH的量。与间接分光光度法测定结果比较，相关性良好；然而，本方法具有几个优点：它更快、更简单且成本更低。对于大量个体，特别是糖尿病患者或白内障患者的山梨醇脱氢酶缺乏筛查应该是有用的。
