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一种用于检测红细胞山梨醇脱氢酶活性的荧光测定法。

A fluorimetric method for red blood cell sorbitol dehydrogenase activity.

作者信息

Vaca G, Zúñiga P, Medina C, Alonso R, González-Quiroga G, Ortíz-De-Luna R I, Cantú J M

出版信息

J Clin Pathol. 1983 Jun;36(6):697-700. doi: 10.1136/jcp.36.6.697.

DOI:10.1136/jcp.36.6.697
PMID:6853734
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC498353/
Abstract

A new fluorimetric method for the quantification of red blood cell (RBC) sorbitol dehydrogenase is described. It is based on the oxidation of sorbitol to fructose, in presence of NAD+, catalysed by the RBC-sorbitol dehydrogenase. The quantity of NADH formed is then measured in a filter fluorimeter. Comparison with an indirect spectrophotometric assay yielded good correlation; however, the present method offers several advantages: it is more rapid, simple and inexpensive. It should be useful to screen for sorbitol dehydrogenase deficiency in large numbers of individuals, particularly patients with diabetes or cataracts.

摘要

本文描述了一种用于定量红细胞山梨醇脱氢酶的新荧光测定法。该方法基于在红细胞山梨醇脱氢酶的催化下，山梨醇在NAD⁺存在的情况下氧化为果糖。然后在滤光荧光计中测量生成的NADH的量。与间接分光光度法测定结果比较，相关性良好；然而，本方法具有几个优点：它更快、更简单且成本更低。对于大量个体，特别是糖尿病患者或白内障患者的山梨醇脱氢酶缺乏筛查应该是有用的。

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A fluorimetric method for red blood cell sorbitol dehydrogenase activity.一种用于检测红细胞山梨醇脱氢酶活性的荧光测定法。
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引用本文的文献

1
Membrane-bound sorbitol dehydrogenase in human red blood cells. Studies in normal subjects and in enzyme-deficient subjects with congenital cataracts.
J Inherit Metab Dis. 1993;16(1):67-72. doi: 10.1007/BF00711317.
2
Sorbitol dehydrogenase deficiency in several pig tissues: potential implications for studies of experimental diabetes.几种猪组织中的山梨醇脱氢酶缺乏症：对实验性糖尿病研究的潜在影响。
Diabetologia. 1984 Oct;27(4):482-3. doi: 10.1007/BF00273916.

本文引用的文献

1
Red blood cell sorbitol dehydrogenase deficiency in a family with cataracts.
Hum Genet. 1982;61(4):338-41. doi: 10.1007/BF00276598.
2
Assignment of the sorbitol dehydrogenase locus to human chromosome 15 pter leads to q21.山梨醇脱氢酶基因座定位于人类染色体15，从染色体短臂末端至长臂的21区带。
Biochem Genet. 1980 Jun;18(5-6):425-31. doi: 10.1007/BF00484391.
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Sorbitol dehydrogenase (EC.1.1.1.14) polymorphism in human seminal plasma.人精浆中山梨醇脱氢酶（EC.1.1.1.14）的多态性
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4
Sorbitol pathway: presence in nerve and cord with substrate accumulation in diabetes.山梨醇途径：存在于神经和脊髓中，在糖尿病时会有底物蓄积。
Science. 1966 Jan 14;151(3707):209-10. doi: 10.1126/science.151.3707.209.
5
New rapid method for the estimation of red cell galactose-1-phosphate uridyl transferase activity.
J Lab Clin Med. 1968 Sep;72(3):527-32.
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Glucose utilization by the polyol pathway in human erythrocytes.人类红细胞中多元醇途径对葡萄糖的利用。
Biochem Biophys Res Commun. 1970 Jul 13;40(1):199-205. doi: 10.1016/0006-291x(70)91066-1.
7
Starch-gel electrophoresis of four enzymes from human red blood cells: glyceraldehyde-3-phosphate dehydrogenase, fructoaldolase, glyoxalase II and sorbitol dehydrogenase.
Ann Hum Genet. 1972 Apr;35(4):477-84. doi: 10.1111/j.1469-1809.1957.tb01873.x.
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The reduction of glyceraldehyde by human erythrocytes. L-hexonate dehydrogenase activity.人红细胞对甘油醛的还原作用。L-己糖酸脱氢酶活性。
J Clin Invest. 1974 May;53(5):1258-64. doi: 10.1172/JCI107672.
9
The role of fructose in restoration of organic phosphate compounds in outdated bank blood.果糖在陈旧库存血中有机磷酸盐化合物恢复中的作用。
J Lab Clin Med. 1973 Sep;82(3):489-99.
10
Metabolic alterations in the human erythrocyte produced by increases in glucose concentration. The role of the polyol pathway.葡萄糖浓度升高引起的人体红细胞代谢改变。多元醇途径的作用。
J Clin Invest. 1971 Oct;50(10):2104-12. doi: 10.1172/JCI106704.

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