Lind P, Løwenstein H
Scand J Immunol. 1983 Mar;17(3):263-73. doi: 10.1111/j.1365-3083.1983.tb00789.x.
An extract of purified Dermatophagoides pteronyssinus mite bodies was investigated by crossed radioimmunoelectrophoresis (CRIE), using sera from 29 mite-allergic patients. Two CRIE series consisting of rabbit antibodies to (1) whole mite culture and to (2) purified mite bodies were run simultaneously, showing 27 and 29 precipitates, respectively. Cases of major discrepancy between the two series were ascribed to absence of antibody to allergenic components and to artefactual radiostaining due to inclusion of allergen in precipitates representing other antigens. Carefully performed controls were necessary to establish the reliability of CRIE. Specific response patterns in the studied set of sera were found to several of the represented antigens. Two major allergens appeared in both CRIE systems. Two intermediate and several minor allergens were further identified.
采用29例螨过敏患者的血清，通过交叉放射免疫电泳（CRIE）对纯化的屋尘螨螨体提取物进行了研究。同时进行了两个CRIE系列，其中兔抗血清分别针对（1）全螨培养物和（2）纯化的螨体，分别显示出27个和29个沉淀带。两个系列之间出现重大差异的情况归因于缺乏针对变应原成分的抗体，以及由于沉淀物中包含代表其他抗原的变应原而导致的人为放射性染色。必须仔细进行对照以确定CRIE的可靠性。在所研究的血清组中发现了对几种所代表抗原的特异性反应模式。在两个CRIE系统中均出现了两种主要变应原。进一步鉴定出两种中间变应原和几种次要变应原。
