Eynard A R, Monis B
J Submicrosc Cytol. 1983 Apr;15(2):375-82.
We studied the ultrastructural distribution of Concanavalin A sites of urothelial and milk fat globule membranes in situ in EFA-deficient and control rats using the peroxidase-diaminobenzidine procedure in unfixed and glutaraldehyde-fixed materials and incubating temperatures of 4 degrees C and 37 degrees C of the Con A media. In the glutaraldehyde ('prefixed') bladders the lectin marker was homogeneously distributed at the surface of the urothelial membrane with no difference between experimental and control animals. In unfixed bladders ('non-prefixed') of EFA-deficient rats, Con A sites distribution was also homogeneous and similar to that observed in 'prefixed' bladders. In bladders of controls in the 'non-prefixed' condition incubated at both 4 degrees and 37 degrees C, there was a noticeable change in the distribution of Con A sites consisting in a distinct clumping of Con A sites. In 'prefixed' and 'non-prefixed' cream samples of both EFA-deficient and control animals, there was a similar, uniform distribution of Con A sites at the surface of the milk fat globule membrane. The reported changes seemed to suggest that the fluidity of the urothelial membrane but not of milk fat globules was diminished in EFA-deficient rats. Present data do not permit evaluation of the role of cytoskeletal components and/or the variations of the fluidity of the membranes on the distribution of Con A sites at cell surfaces, as herein reported.
我们采用过氧化物酶 - 二氨基联苯胺法，在未固定和戊二醛固定的材料中，以及在4℃和37℃的伴刀豆球蛋白A（Con A）培养基孵育温度下，研究了必需脂肪酸（EFA）缺乏和对照大鼠原位尿路上皮及乳脂肪球膜上Con A位点的超微结构分布。在戊二醛（“预固定”）膀胱中，凝集素标记物均匀分布于尿路上皮膜表面，实验动物和对照动物之间无差异。在EFA缺乏大鼠的未固定膀胱（“非预固定”）中，Con A位点分布也是均匀的，且与“预固定”膀胱中观察到的相似。在4℃和37℃孵育的“非预固定”状态的对照膀胱中，Con A位点的分布有明显变化，表现为Con A位点明显聚集。在EFA缺乏和对照动物的“预固定”和“非预固定”奶油样品中，乳脂肪球膜表面Con A位点有相似的均匀分布。所报道的这些变化似乎表明，EFA缺乏大鼠尿路上皮膜的流动性降低，而乳脂肪球膜的流动性未降低。本文报道的现有数据无法评估细胞骨架成分的作用和/或膜流动性变化对细胞表面Con A位点分布的影响。
