Kalinec F, Calderón J H, Monis B
J Histochem Cytochem. 1986 Dec;34(12):1639-44. doi: 10.1177/34.12.3782776.
The present report deals with a densitometric study of the ultrastructural images of the urothelial membrane of rats in the following experimental conditions: (1) EFA-deficient (EFAD) rats; (2) EFA-sufficient (EFAS) rats; and (3) EFAD rats that were fed the EFAD diet for 30 weeks and received an EFAS diet for the following 10 weeks (EFAD/S group). On electron micrographs of the transitional epithelium of ureters and urinary bladder of these rats, optical density (OD) profiles of the urothelial unit membrane were recorded and digitized using a computer-controlled microdensitometer with a solid-state self-scanned photodiode array sensor. A Gaussian curve was adopted as a model for the distribution of electron-dense material in each osmiophilic leaflet. Gaussian parameters were used to estimate the thickness of the urothelial membrane and of each osmiophilic leaflet, and the amount of electron-dense material and the maximal electron density present in each leaflet. In EFAS rats, the thick urothelial membrane was asymmetric like that of the normal, resulting from a greater thickness of the outer leaflet and a greater electron density of the cytoplasmic one. In EFAD rats, a loss of the characteristic ultrastructural asymmetry and a decrease of the total thickness of the unit membrane were detected. These changes were partially reversed in the EFAD/S rats.
（1）必需脂肪酸缺乏（EFAD）大鼠；（2）必需脂肪酸充足（EFAS）大鼠；以及（3）先给予EFAD饮食30周，随后给予EFAS饮食10周的EFAD大鼠（EFAD/S组）。在这些大鼠输尿管和膀胱移行上皮的电子显微镜照片上，使用配备固态自扫描光电二极管阵列传感器的计算机控制显微密度计记录尿路上皮单位膜的光密度（OD）轮廓并进行数字化处理。采用高斯曲线作为每个嗜锇小叶中电子致密物质分布的模型。高斯参数用于估计尿路上皮膜和每个嗜锇小叶的厚度，以及每个小叶中电子致密物质的含量和最大电子密度。在EFAS大鼠中，厚的尿路上皮膜与正常情况一样不对称，这是由于外层小叶厚度更大且细胞质小叶的电子密度更高所致。在EFAD大鼠中，检测到特征性超微结构不对称性丧失以及单位膜总厚度减小。这些变化在EFAD/S大鼠中部分得到逆转。
