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通过禽类α-珠蛋白基因座的DNA复制极性

Polarity of DNA replication through the avian alpha-globin locus.

作者信息

James C D, Leffak M

出版信息

Mol Cell Biol. 1986 Apr;6(4):976-84. doi: 10.1128/mcb.6.4.976-984.1986.

DOI:10.1128/mcb.6.4.976-984.1986
PMID:3785167
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367605/
Abstract

Toward understanding the controls affecting eucaryotic chromosome replication, we used a runoff replication assay to investigate whether the activity of a gene is related to its use of an upstream or downstream replication origin. When in vivo-initiated DNA polymerases are allowed to complete replication in vitro in the presence of bromodeoxyuridine triphosphate the density label is preferentially incorporated into origin-distal regions of DNA. Isopycnic centrifugation and blot hybridization analysis of the relative bromodeoxyuridine triphosphate incorporation into fragments spanning the chicken alpha-globin locus indicate that this region is replicated from an upstream origin both in chicken lymphocytes and in erythrocytes. Thus the replication polarity of these genes does not change as a function of transcriptional activity, consistent with earlier suggestions that DNA replication in the transcriptional direction may be a necessary but not sufficient condition for gene expression.

摘要

为了理解影响真核染色体复制的调控机制，我们采用了连续复制分析方法来研究基因的活性是否与其对上游或下游复制起点的利用有关。当体内启动的DNA聚合酶在溴脱氧尿苷三磷酸存在的情况下于体外完成复制时，密度标记会优先掺入到DNA的起点远端区域。对跨越鸡α-珠蛋白基因座的片段进行等密度离心和印迹杂交分析，结果表明该区域在鸡淋巴细胞和红细胞中均从上游起点开始复制。因此，这些基因的复制极性不会随转录活性而改变，这与早期的观点一致，即沿转录方向进行DNA复制可能是基因表达的必要但非充分条件。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc63/367605/8d9af4bb0d08/molcellb00088-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc63/367605/219e6752789e/molcellb00088-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc63/367605/84744eabccc2/molcellb00088-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc63/367605/2a4aa2b78e01/molcellb00088-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc63/367605/717edd1ba8fc/molcellb00088-0023-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc63/367605/8d9af4bb0d08/molcellb00088-0024-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc63/367605/219e6752789e/molcellb00088-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc63/367605/84744eabccc2/molcellb00088-0022-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc63/367605/2a4aa2b78e01/molcellb00088-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc63/367605/717edd1ba8fc/molcellb00088-0023-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc63/367605/8d9af4bb0d08/molcellb00088-0024-a.jpg

相似文献

1
Polarity of DNA replication through the avian alpha-globin locus.通过禽类α-珠蛋白基因座的DNA复制极性
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DNase-sensitive chromatin structure near a chromosomal origin of bidirectional replication of the avian alpha-globin locus.禽类α-珠蛋白基因座双向复制染色体起源附近的DNase敏感染色质结构。
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4
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Alteration of in vitro DNA synthesis in the alpha globin locus of chick embryo fibroblasts due to in vivo activity of Rous sarcoma virus pp60src.由于劳氏肉瘤病毒pp60src的体内活性，鸡胚成纤维细胞α珠蛋白基因座的体外DNA合成发生改变。
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Reactivation of chicken erythrocyte nuclei in heterokaryons results in expression of adult chicken globin genes.异核体中鸡红细胞核的重新激活导致成年鸡珠蛋白基因的表达。
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Analysis of the replication direction through the domain of alpha-globin-encoding chicken genes.
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[Localization of regions of DNA attachment to the nuclear skeleton within chicken alpha-globin genes in functionally active and functionally inactive nuclei].[在功能活跃和功能不活跃细胞核内鸡α-珠蛋白基因中与核骨架相连的DNA区域的定位]
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The replication timing program of the Chinese hamster beta-globin locus is established coincident with its repositioning near peripheral heterochromatin in early G1 phase.中国仓鼠β-珠蛋白基因座的复制时间程序是在早期G1期其重新定位到外周异染色质附近时建立的。
J Cell Biol. 2001 Jul 23;154(2):283-92. doi: 10.1083/jcb.200104043.

引用本文的文献

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Transcription factor binding and induced transcription alter chromosomal c-myc replicator activity.转录因子结合与诱导转录改变染色体c-myc复制子活性。
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Characterizing replication intermediates in the amplified CHO dihydrofolate reductase domain by two novel gel electrophoretic techniques.

本文引用的文献

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Replication and recombination functions associated with the yeast plasmid, 2 mu circle.与酵母质粒2μm环相关的复制和重组功能。
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Alteration of in vivo DNA synthesis in the alpha globin locus of chick embryo fibroblasts due to in vivo activity of Rous sarcoma virus pp60src.由于劳氏肉瘤病毒pp60src的体内活性，鸡胚成纤维细胞α珠蛋白基因座的体内DNA合成发生改变。
Nucleic Acids Res. 1994 Feb 11;22(3):498-505.
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Two alleles of a developmentally regulated alpha-tubulin locus in Physarum polycephalum replicate on different schedules.多头绒泡菌中一个受发育调控的α-微管蛋白基因座的两个等位基因按不同的时间表复制。
Mol Cell Biol. 1993 Jan;13(1):449-61. doi: 10.1128/mcb.13.1.449-461.1993.
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Two early replicated, developmentally controlled genes of Physarum display different patterns of DNA replication by two-dimensional agarose gel electrophoresis.绒泡菌的两个早期复制、发育调控基因通过二维琼脂糖凝胶电泳显示出不同的DNA复制模式。
Chromosoma. 1993 Sep;102(8):563-74. doi: 10.1007/BF00368349.
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Mapping of replication initiation sites in human ribosomal DNA by nascent-strand abundance analysis.通过新生链丰度分析绘制人类核糖体DNA中的复制起始位点图谱。
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Nuclear DNA synthesis in vitro is mediated via stable replication forks assembled in a temporally specific fashion in vivo.体外核DNA合成是通过在体内以时间特异性方式组装的稳定复制叉介导的。
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Opposite replication polarities of transcribed and nontranscribed histone H5 genes.转录的和未转录的组蛋白H5基因的相反复制极性
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Role of cytosol proteins in DNA chain growth and chromatin replication in Friend erythroleukemia cell nuclei.胞质溶胶蛋白在Friend红白血病细胞核中DNA链生长和染色质复制中的作用
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Origin of replication from Xenopus laevis mitochondrial DNA promotes high-frequency transformation of yeast.非洲爪蟾线粒体DNA的复制起点促进酵母的高频转化。
Proc Natl Acad Sci U S A. 1981 May;78(5):3128-32. doi: 10.1073/pnas.78.5.3128.
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Autonomously replicating sequences in Saccharomyces cerevisiae.酿酒酵母中的自主复制序列。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6329-33. doi: 10.1073/pnas.77.11.6329.
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An amplified chromosomal sequence that includes the gene for dihydrofolate reductase initiates replication within specific restriction fragments.一个包含二氢叶酸还原酶基因的扩增染色体序列在特定限制片段内启动复制。
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4083-7. doi: 10.1073/pnas.79.13.4083.
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Replication of alpha and beta globin DNA sequences occurs during early S phase in murine erythroleukemia cells.α和β珠蛋白DNA序列的复制发生在小鼠红白血病细胞的S期早期。
Proc Natl Acad Sci U S A. 1981 May;78(5):3058-62. doi: 10.1073/pnas.78.5.3058.
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Varigated chromatin structures of mouse ribosomal RNA genes.小鼠核糖体RNA基因的斑驳染色质结构。
J Mol Biol. 1983 Jun 15;167(1):133-55. doi: 10.1016/s0022-2836(83)80038-2.
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Stability of the conservative mode of nucleosome assembly.核小体组装保守模式的稳定性。
Nucleic Acids Res. 1983 May 11;11(9):2717-32. doi: 10.1093/nar/11.9.2717.