Fóris G, Boján F, Medgyesi G A, Szilágyi T
Agents Actions. 1983 Feb;13(1):63-8. doi: 10.1007/BF01994284.
Effects of urethan on some functions of blood lymphocytes and peritoneal macrophages (PMs) of rats were studied in in vivo and in vitro experiments. The in vitro lymphokine (LK) producing activity of lymphocytes in the presence of specific antigen was depressed by urethan administered 1-5 days before the BCG sensitization. However, the drug injected after the BCG sensitization was not effective on the LK production. Urethan added to the cultures of previously BCG-primed lymphocytes did not influence the LK production. The sensitivity of glycogen-provoked PMs (pPM) to LK-induced activation and, at the same time, the 125I-IgG2a binding capacity as well as the EA rosette formation of the provoked PMs were depressed by urethan administered 1-5 days before the lavage of PMs. These functions of resident PMs (rPM) were not altered by the drug treatment. Urethan added to the cultures of resident or provoked PMs proved to be ineffective. These results led to the conclusions that urethan, after its in vivo metabolic conversion, causes an impairment of the macrophage functions in the inductive phase of the immune response and this event may be the crucial point in the immunosuppressive effect of urethan.
在体内和体外实验中研究了乌拉坦对大鼠血液淋巴细胞和腹腔巨噬细胞（PMs）某些功能的影响。在卡介苗致敏前1 - 5天给予乌拉坦，可抑制淋巴细胞在特异性抗原存在下体外产生淋巴因子（LK）的活性。然而，在卡介苗致敏后注射该药物对LK产生无效。将乌拉坦添加到先前经卡介苗致敏的淋巴细胞培养物中不影响LK产生。在冲洗PMs前1 - 5天给予乌拉坦，可降低糖原激发的PMs（pPM）对LK诱导激活的敏感性，同时降低激发的PMs的125I - IgG2a结合能力以及EA花环形成。常驻PMs（rPM）的这些功能未因药物处理而改变。将乌拉坦添加到常驻或激发的PMs培养物中证明无效。这些结果得出结论，乌拉坦在体内代谢转化后，在免疫反应的诱导阶段导致巨噬细胞功能受损，这一事件可能是乌拉坦免疫抑制作用的关键点。
