肉碱棕榈酰转移酶的滞后行为。丙二酰辅酶A预温育的影响。
Hysteretic behaviour of carnitine palmitoyltransferase. The effect of preincubation with malonyl-CoA.
作者信息
Cook G A, Cox K A
出版信息
Biochem J. 1986 Jun 15;236(3):917-9. doi: 10.1042/bj2360917.
Abstract
Continuous assays of carnitine palmitoyltransferase were used to study the hysteretic behaviour of the enzyme. When reactions were started by adding mitochondria to complete reaction mixtures, there was a lag in the assay even in the absence of malonyl-CoA. When mitochondria were preincubated with malonyl-CoA in the absence of palmitoyl-CoA, there was a greater lag period in the assay of carnitine palmitoyltransferase, but this lag was less prominent at 37 degrees C than at 30 degrees C. Preincubation of mitochondria with malonyl-CoA did not change the sensitivity of the enzyme to inhibition by malonyl-CoA.
摘要
采用肉碱棕榈酰转移酶连续测定法来研究该酶的滞后行为。当通过向完整反应混合物中添加线粒体来启动反应时，即使在不存在丙二酰辅酶A的情况下，测定中也存在滞后现象。当线粒体在不存在棕榈酰辅酶A的情况下与丙二酰辅酶A预孵育时，肉碱棕榈酰转移酶测定中的滞后时间更长，但这种滞后在37℃时比在30℃时不那么明显。线粒体与丙二酰辅酶A预孵育不会改变该酶对丙二酰辅酶A抑制的敏感性。
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