Banville D L, Marzilli L G, Wilson W D
Biochem Biophys Res Commun. 1983 May 31;113(1):148-54. doi: 10.1016/0006-291x(83)90444-8.
The interactions of meso-tetra(4-N-methylpyridyl) porphine (TMPyP) and its Zn(II) and Ni(II) derivatives with DNA have been investigated by 31P NMR and viscometric titrations. TMPyP and its Ni derivative increase the viscosity of linear DNA, cause unwinding and reverse coiling of superhelical DNA, and induce a separate downfield peak in the 31P NMR spectrum of DNA. The Zn derivative slightly decreases the viscosity of linear DNA, does not unwind superhelical DNA, and does not give a downfield NMR peak. The main DNA 31P NMR signal is shifted slightly upfield on either the addition of TMPyP or the Ni compound. These results indicate that TMPyP and the Ni(II), but not the Zn(II), derivative bind to DNA by intercalation.
通过31P核磁共振和粘度滴定法研究了中-四（4-N-甲基吡啶基）卟啉（TMPyP）及其锌（II）和镍（II）衍生物与DNA的相互作用。TMPyP及其镍衍生物增加线性DNA的粘度，导致超螺旋DNA解旋和反向卷曲，并在DNA的31P核磁共振谱中诱导出一个单独的低场峰。锌衍生物略微降低线性DNA的粘度，不解旋超螺旋DNA，也不产生低场核磁共振峰。添加TMPyP或镍化合物后，主要的DNA 31P核磁共振信号略微向高场移动。这些结果表明，TMPyP和镍（II）衍生物而非锌（II）衍生物通过嵌入作用与DNA结合。
