Carvlin M J, Fiel R J
Nucleic Acids Res. 1983 Sep 10;11(17):6121-39. doi: 10.1093/nar/11.17.6121.
The large meso-substituted porphine, meso-tetra(4-N-methylpyridyl)porphine has been identified as a DNA-interactive ligand with a capacity for intercalation (1,2). Subsequently, the 2-N-methyl, 3-N-methyl and N-trimethylanilinium analogues of this porphyrin intercalator have been obtained for physico-chemical analyses (absorption spectroscopy, viscometry, circular dichroism, unwinding of supercoiled DNA). In this paper we discuss the factors affecting the character of porphyrin binding (intercalative, as is the case for the 4-N-methyl and 3-N-methyl porphines, versus non-intercalative, as is the case for the 2-N-methyl and N-trimethylanilinium porphines) and the impact that porphyrins' binding has upon the structure of DNA. The molecular conformation of the porphyrin ligand varies slightly within this series so that the ability of a given porphyrin to intercalate may be correlated with the arrangement of charged groups, the planarity of the porphine ring and the effective width of the individual molecules. The results from these studies indicate that sequence selective binding occurs within a small aperture of solution conditions.
大的中位取代卟啉，即中位四（4 - N - 甲基吡啶基）卟啉，已被确定为一种具有嵌入能力的DNA相互作用配体（1,2）。随后，已获得该卟啉嵌入剂的2 - N - 甲基、3 - N - 甲基和N - 三甲基苯胺类似物用于物理化学分析（吸收光谱、粘度测定、圆二色性、超螺旋DNA解旋）。在本文中，我们讨论了影响卟啉结合特性的因素（对于4 - N - 甲基和3 - N - 甲基卟啉是嵌入性的，而对于2 - N - 甲基和N - 三甲基苯胺卟啉是非嵌入性的）以及卟啉结合对DNA结构的影响。卟啉配体的分子构象在该系列中略有变化，因此给定卟啉的嵌入能力可能与带电基团的排列、卟啉环的平面性以及单个分子的有效宽度相关。这些研究结果表明，在溶液条件的一个小范围内会发生序列选择性结合。
