体外分离的成年大鼠少突胶质细胞生长增强及形态分化：天然产生的细胞外基质的应用

Enhanced growth and morphological differentiation of isolated adult rat oligodendrocytes in vitro: use of a naturally produced extracellular matrix.

作者信息

Lubetzki-Korn I, Ovadia H, Vlodavsky I, Fuks Z, Abramsky O

出版信息

Brain Res. 1983 May 9;267(1):151-5. doi: 10.1016/0006-8993(83)91049-1.

Abstract

Attachment, growth and morphological differentiation of isolated adult rat oligodendrocytes cultured on a naturally produced basement membrane-like extracellular matrix (ECM) occurred much faster than on poly-L-lysine (PLL) coated tissue culture dishes. In each individual trial the cells cultured on ECM exhibited, within 3-5 days in culture, a massive outgrowth of long and branched cytoplasmic processes. Outgrowth to such an extent, using PLL or plastic tissue culture dishes, was not observed even after 2 weeks in culture. The successful high plating efficiency, rapid growth and network formation as well as its resemblance to the in vivo environment of cells make this naturally produced substrate a superior substitute for PLL and therefore more attractive for studying the behavior and function of oligodendrocytes in vitro.

摘要

在天然生成的类似基底膜的细胞外基质（ECM）上培养的成年大鼠少突胶质细胞的附着、生长和形态分化，比在涂有聚-L-赖氨酸（PLL）的组织培养皿上发生得快得多。在每个单独的试验中，在ECM上培养的细胞在培养3-5天内，就出现了大量长而分支的细胞质突起的生长。使用PLL或塑料组织培养皿，即使在培养2周后也未观察到如此程度的生长。这种天然生成的底物具有成功的高接种效率、快速生长和网络形成，以及与细胞体内环境的相似性，使其成为PLL的优质替代品，因此对于体外研究少突胶质细胞的行为和功能更具吸引力。