Gallagher K P, Matsuzaki M, Osakada G, Kemper W S, Ross J
Circ Res. 1983 Jun;52(6):716-29. doi: 10.1161/01.res.52.6.716.
Relationships between regional myocardial perfusion and transmural function, both during treadmill exercise and at rest, were examined in conscious dogs with varying degrees of coronary stenosis produced by a hydraulic occluder. In 13 dogs we measured myocardial blood flow with microspheres (10-12 microns in diameter) and regional systolic wall thickening (%). During exercise with coronary stenosis, myocardial blood flow was characterized by nonuniform distribution, and associated with regional dysfunction. The relationships between normalized myocardial blood flow and normalized %wall thickening during exercise with coronary stenosis were linear, with significantly different slopes (mean myocardial blood flow: y = 1.23x - 0.16, r = 0.93; subendocardial myocardial blood flow: y = 1.50x - 0.02, r = 0.86; subepicardial myocardial blood flow: y = 0.83x - 0.18, r = 0.87). To fill the gap between available subendocardial and subepicardial data during exercise with coronary stenosis and control points, however, would require nonlinear components. In 10 of the dogs, coronary stenosis at rest was also produced to compare regional myocardial blood flow - %wall thickening relations at rest with those during steady state exercise. The absolute mean myocardial blood flow - %wall thickening relation during exercise with coronary stenosis (y = 11.6x - 1.9, r = 0.90) was significantly shifted rightward from the resting relation (y = 25.3x -2.1, r = 0.80). However, when changes in %wall thickening were plotted vs. myocardial blood flow per beat, the relationships at rest and exercise were nearly superimposable. Likewise, relations between normalized myocardial blood flow and changes in %wall thickening at rest and exercise were not significantly different. We conclude: %wall thickening during exercise is directly related to changes in mean myocardial blood flow but is related in nonlinear fashion to changes in subepicardial and subendocardial myocardial blood flow; %wall thickening may provide a reliable index of the relative transmural flow distribution during exercise as well as at rest; during brief bouts (5-8 minutes) of exercise with coronary stenosis, the relationship between stabilized regional contractile dysfunction and level of myocardial blood flow per beat is the same as that during coronary stenosis at rest.
在有意识的犬类中，通过液压闭塞器制造出不同程度的冠状动脉狭窄，以此研究跑步机运动期间及静息时局部心肌灌注与透壁功能之间的关系。在13只犬中，我们用微球（直径10 - 12微米）测量心肌血流量，并测量局部收缩期室壁增厚（%）。在冠状动脉狭窄的运动过程中，心肌血流量表现为分布不均匀，并伴有局部功能障碍。冠状动脉狭窄运动期间，标准化心肌血流量与标准化室壁增厚百分比之间的关系呈线性，斜率显著不同（平均心肌血流量：y = 1.23x - 0.16，r = 0.93；心内膜下心肌血流量：y = 1.50x - 0.02，r = 0.86；心外膜下心肌血流量：y = 0.83x - 0.18，r = 0.87）。然而，为了填补冠状动脉狭窄运动期间可用的心内膜下和心外膜下数据与对照点之间的差距，将需要非线性成分。在10只犬中，还制造了静息时的冠状动脉狭窄，以比较静息时局部心肌血流量与室壁增厚百分比的关系以及稳态运动期间的关系。冠状动脉狭窄运动期间的绝对平均心肌血流量与室壁增厚百分比关系（y = 11.6x - 1.9，r = 0.90）相对于静息时的关系（y = 25.3x - 2.1，r = 0.80）显著右移。然而，当将室壁增厚百分比的变化与每搏心肌血流量作图时，静息和运动时的关系几乎重叠。同样，静息和运动时标准化心肌血流量与室壁增厚百分比变化之间的关系无显著差异。我们得出结论：运动期间的室壁增厚百分比与平均心肌血流量的变化直接相关，但与心外膜下和心内膜下心肌血流量的变化呈非线性关系；室壁增厚百分比可提供运动及静息期间相对透壁血流分布的可靠指标；在冠状动脉狭窄的短暂运动（5 - 8分钟）期间，稳定的局部收缩功能障碍与每搏心肌血流量水平之间的关系与冠状动脉狭窄静息时相同。
