Mössner E, Pfleiderer G
Clin Biochem. 1983 Feb;16(1):28-30. doi: 10.1016/s0009-9120(83)94330-8.
Antibodies against placental alkaline phosphatase (PAP) share antigenic determinants with the intestinal isoenzyme (IAP) and vice versa. Both isoenzymes can be found as part of the total activity of alkaline phosphatase (AP) in the serum. Using antibody-coated polystyrene tubes, a simple and sensitive immunoassay was developed which allows the quantitative determination of IAP or PAP without interference of the cross-reacting isoenzyme. The presence and amount of IAP depends on the ABO blood group, secretory status and the oral fat intake. The serum IAP in healthy fasted individuals was found up to 8 U/I in secretors of blood group O and B and below 1 U/I in non-secretors and blood group A donors. In screening tests of various pathological sera. IAP was found elevated up to 100 U/I in idiopathic hyper-AP-aemia and some liver cirrhosis patients.
抗胎盘碱性磷酸酶（PAP）的抗体与肠型同工酶（IAP）具有共同的抗原决定簇，反之亦然。这两种同工酶均可在血清碱性磷酸酶（AP）的总活性中检测到。利用抗体包被的聚苯乙烯管，开发了一种简单且灵敏的免疫测定方法，可对IAP或PAP进行定量测定，而不受交叉反应同工酶的干扰。IAP的存在及含量取决于ABO血型、分泌状态和口服脂肪摄入量。在健康禁食个体中，O型和B型血分泌者的血清IAP高达8 U/I，非分泌者和A型血供血者的血清IAP低于1 U/I。在对各种病理血清的筛查试验中，发现特发性高AP血症和一些肝硬化患者的IAP升高至100 U/I。
