Pearson C D, McLean S A, Tetley K, Phillips R S
Clin Exp Immunol. 1983 Apr;52(1):121-8.
Spleen cells and peripheral blood mononuclear cells (PBMC) from mice, which had recovered from infection with Plasmodium chabaudi, were induced to produce anti-P. chabaudi antibody by incubating the cells with P. chabaudi parasitized red cells in Marbrook cultures. The anti-malarial antibody was assayed using the indirect fluorescent antibody test. Spleen cells and PBMC from mice infected 2-4 months previously gave higher antibody titres in culture than similar cells from mice infected a year previously. There was a good correlation between the ability of spleen cells or PBMC to be stimulated to produce antibody in vitro and the ability of mice similar to the cell donors to resist a challenge infection. Some immunity can be adoptively transferred with both spleen cells and PBMC.
从感染恰氏疟原虫后已康复的小鼠中获取脾细胞和外周血单个核细胞（PBMC），将这些细胞与恰氏疟原虫寄生的红细胞在马尔布鲁克培养物中共同孵育，诱导其产生抗恰氏疟原虫抗体。使用间接荧光抗体试验检测抗疟抗体。与感染一年前的小鼠的类似细胞相比，感染2 - 4个月前的小鼠的脾细胞和PBMC在培养中产生的抗体滴度更高。脾细胞或PBMC在体外被刺激产生抗体的能力与类似于细胞供体的小鼠抵抗攻击感染的能力之间存在良好的相关性。部分免疫力可通过脾细胞和PBMC进行过继转移。
