Meddens M J, Thompson J, Bauer W C, Hermans J, van Furth R
Infect Immun. 1983 Jul;41(1):145-53. doi: 10.1128/iai.41.1.145-153.1983.
The role of granulocytes and monocytes during the induction and course of Staphylococcus epidermidis endocarditis was investigated by the selective depletion of monocytes with the drug VP16-213 and of both granulocytes and monocytes with nitrogen mustard. The induction of endocarditis was influenced only by the depletion of monocytes: the 50% infective dose differed significantly, being 3.4 X 10(5) CFU in control rabbits and 3.4 X 10(4) CFU in the monocyte-depleted rabbits, whereas no significant differences were found between the latter and those depleted of both granulocytes and monocytes. Also, control rabbits injected with 10(6) or 10(7) CFU had a significantly higher incidence of sterile vegetations than did rabbits selectively depleted of granulocytes or monocytes. Compared with baseline values, mean monocyte numbers at the time of bacterial inoculation were significantly increased in control rabbits whose vegetations remained sterile, whereas this effect was not seen in rabbits whose vegetations became infected. The course of the endocarditis appeared to be significantly influenced by both granulocytes and monocytes. Comparison showed that a decrease of the same numbers of these cells per microliter of blood was accompanied for the monocytes by an approximately fourfold higher increase of the number of staphylococci in the vegetations. The correlation between the number of granulocytes and of monocytes on the one hand and the number of staphylococci in the vegetations on the other was not substantially influenced by the duration of the disease or the number of staphylococci injected to induce the endocarditis. The number injected proved to be significantly correlated with the number of staphylococci in the vegetations. In rabbits with numbers of CFU per gram of vegetation exceeding 10(7), blood cultures were usually positive. This finding applied rarely to control rabbits, but generally to drug-treated rabbits. In the latter animals a significant correlation between the number of staphylococci in the vegetations and in the circulation was found. We conclude that only monocytes have a measurable effect on the induction of Staphylococcus epidermidis endocarditis but during its course both granulocytes and monocytes keep the endocardial infection in check.
通过用药物VP16 - 213选择性清除单核细胞以及用氮芥清除粒细胞和单核细胞，研究了粒细胞和单核细胞在表皮葡萄球菌心内膜炎的诱发及病程中的作用。心内膜炎的诱发仅受单核细胞清除的影响：半数感染剂量有显著差异，对照兔为3.4×10⁵CFU，单核细胞清除兔为3.4×10⁴CFU，而单核细胞清除兔与粒细胞和单核细胞均清除的兔之间未发现显著差异。此外，注射10⁶或10⁷CFU的对照兔无菌赘生物的发生率显著高于选择性清除粒细胞或单核细胞的兔。与基线值相比，赘生物保持无菌的对照兔在细菌接种时的平均单核细胞数量显著增加，而赘生物被感染的兔未观察到这种效应。心内膜炎的病程似乎受粒细胞和单核细胞两者的显著影响。比较表明，每微升血液中相同数量的这些细胞减少时，单核细胞减少伴随赘生物中葡萄球菌数量增加约四倍。粒细胞和单核细胞数量与赘生物中葡萄球菌数量之间的相关性基本上不受疾病持续时间或诱发心内膜炎所注射葡萄球菌数量的影响。所注射的数量与赘生物中葡萄球菌数量显著相关。每克赘生物CFU数量超过10⁷的兔，血培养通常为阳性。这一发现很少适用于对照兔，但普遍适用于药物治疗的兔。在后者动物中，发现赘生物和循环中葡萄球菌数量之间存在显著相关性。我们得出结论，只有单核细胞对表皮葡萄球菌心内膜炎的诱发有可测量的影响，但在其病程中，粒细胞和单核细胞都能控制心内膜感染。
