采用酶联免疫吸附测定法检测抗金黄色葡萄球菌磷壁酸抗体。
Detection of antibody to Staphylococcus aureus teichoic acid by enzyme-linked immunosorbent assay.
作者信息
Yamada J K, Inderlied C B, Porschen R K
出版信息
J Clin Microbiol. 1983 May;17(5):898-905. doi: 10.1128/jcm.17.5.898-905.1983.
Abstract
A sensitive, specific, and rapid enzyme-linked immunosorbent assay has been developed for the detection of immunoglobulin G to Staphylococcus aureus teichoic acid in human sera. Detection of S. aureus teichoic acid antibody is at least 800 times more sensitive than a double diffusion in gel assay, and positive titers of 1:25,600 and greater were observed with this assay. Results with the enzyme-linked immunosorbent assay can be obtained within 3.5 h by using antigen-coated cuvettes. Quantitation of S. aureus teichoic acid antibody by this enzyme-linked immunosorbent assay may be useful in the initial as well as the follow-up diagnosis of serious S. aureus infections.
摘要
已开发出一种灵敏、特异且快速的酶联免疫吸附测定法，用于检测人血清中针对金黄色葡萄球菌磷壁酸的免疫球蛋白G。检测金黄色葡萄球菌磷壁酸抗体的灵敏度至少比凝胶双扩散测定法高800倍，该测定法观察到的阳性效价为1:25,600及以上。使用包被抗原的比色皿，可在3.5小时内获得酶联免疫吸附测定的结果。通过这种酶联免疫吸附测定法定量金黄色葡萄球菌磷壁酸抗体，可能有助于严重金黄色葡萄球菌感染的初始诊断以及后续诊断。
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本文引用的文献
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